Lamin B1 antibody [mAbcam 28129] (ab28129)

Product Name 

Lamin B1 antibody [mAbcam 28129]

Product type 

Primary antibodies

Description 

Mouse monoclonal [mAbcam 28129] to Lamin B1

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Lamin B1.

(Peptide available as ab16375.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Chk, Cow, Zfsh
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate.

Cellular localization 

Nuclear membrane

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class V >> Lamins
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Envelope
Cell Biology >> Apoptosis >> Nucleus >> Lamins

Relevance 

An important part of the nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of alpha helices and a carboxy terminal globular domain.
Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus.
Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organised into loops, which are attached to the nuclear matrix. This organisation is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A and B type lamins.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

mAbcam 28129

Myeloma 

Sp2/0

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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