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- Secondary antibodies
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Product name
Anti-Laminin 5 antibody
See all Laminin 5 products (7) ...
Description
Rabbit polyclonal to Laminin 5
Tested applications
IP, ICC/IF, IHC-Fr, WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Horse, Dog, Human
Immunogen
Purified Human Laminin 5 protein.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.2% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. PubMed: 16940506
ICC/IF: Use at an assay dependent dilution. PubMed: 16940506
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 165, 140, 105 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
Tissue specificity
Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.
Involvement in disease
Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS) [MIM:245660]. LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx.
Sequence similarities
Contains 15 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
Target information above from: UniProt accessionQ16787
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BM600 antibody
- E170 antibody
- EBR2A antibody
see all
Database links
- Entrez Gene: 3914 Human
- Entrez Gene: 3918 Human
- Entrez Gene: 3909 Human
- Omim: 150310 Human
- Omim: 226650 Human
- Omim: 226700 Human
- SwissProt: Q13751 Human
- SwissProt: Q13753 Human
see all
Anti-Laminin 5 antibody images:
Immunocytochemistry/ Immunofluorescence - Anti-Laminin 5 antibody (ab14509)
ICC/IF image of ab14509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Laminin 5 antibody (ab14509)
This product has been referenced in:
- Sarkar TR et al. Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPd expression and contributes to transformation of breast tumor cells. Mol Cell Biol 32:320-32 (2012). ICC/IF; Human.Read more (PubMed: 22037769) »
- Dasgupta J et al. Structural basis of oligosaccharide receptor recognition by human papillomavirus. J Biol Chem 286:2617-24 (2011). ICC/IF; Human.Read more (PubMed: 21115492) »
See all 13 publications for this product
Publishing research using ab14509? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"