Anti-Laminin 5 antibody (ab14509)
Key features and details
- Rabbit polyclonal to Laminin 5
- Suitable for: WB, ELISA, IHC-Fr, ICC/IF, IP
- Reacts with: Mouse, Horse, Dog, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Laminin 5 antibody
See all Laminin 5 primary antibodies -
Description
Rabbit polyclonal to Laminin 5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, IHC-Fr, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Horse, Dog, Human -
Immunogen
This product was produced with the following immunogens:
Full length native protein (purified) corresponding to Human Laminin 5. Produced against native human Laminin 5, isolated from ceratynocytes primary culture material. Swiss Prot Q16787, Q13751 and Q13753.
Database link: Q16787
Full length native protein (purified) corresponding to Human Laminin 5. Produced against native human Laminin 5, isolated from ceratynocytes primary culture material. Swiss Prot Q16787, Q13751 and Q13753.
Database link: Q13751
Full length native protein (purified) corresponding to Human Laminin 5. Produced against native human Laminin 5, isolated from ceratynocytes primary culture material. Swiss Prot Q16787, Q13751 and Q13753.
Database link: Q13753 -
Positive control
- WB: Cell culture supernatant of keratinocytes. HaCaT cell supernatant. SCC-25 cell supernatant. Purified Laminin 5.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.2% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
Use at an assay dependent concentration. Detects a band of approximately 165, 140, 105 kDa.
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ELISA | (1) |
Use at an assay dependent concentration.
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IHC-Fr | (4) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 16940506
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IP |
Use at an assay dependent concentration. PubMed: 16940506
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 165, 140, 105 kDa. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 16940506 |
IP
Use at an assay dependent concentration. PubMed: 16940506 |
Target
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Relevance
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. -
Database links
- Entrez Gene: 3909 Human
- Entrez Gene: 3914 Human
- Entrez Gene: 3918 Human
- SwissProt: Q13751 Human
- SwissProt: Q13753 Human
- SwissProt: Q16787 Human
- SwissProt: Q61087 Mouse
- SwissProt: Q61092 Mouse
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (67)
ab14509 has been referenced in 67 publications.
- Mezawa M et al. TNF-α regulates the composition of the basal lamina and cell-matrix adhesions in gingival epithelial cells. Cell Adh Migr 16:13-24 (2022). PubMed: 35137648
- Jones CFE et al. Design of an Integrated Microvascularized Human Skin-on-a-Chip Tissue Equivalent Model. Front Bioeng Biotechnol 10:915702 (2022). PubMed: 35928950
- Ali G & Abdelalim EM Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells. STAR Protoc 3:101613 (2022). PubMed: 35990735
- Melcher ML et al. Interplay of the transcription factor MRTF-A and matrix stiffness controls mammary acinar structure and protrusion formation. Cell Commun Signal 20:158 (2022). PubMed: 36229824
- Parigoris E et al. Cancer Cell Invasion of Mammary Organoids with Basal-In Phenotype. Adv Healthc Mater 10:e2000810 (2021). PubMed: 32583612