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Anti-Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)

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Overview

Product name

Anti-Laminin B2 gamma 1 antibody [A5] - BSA and Azide free
See all Laminin B2 gamma 1 products (4) ...

Description

Rat monoclonal [A5] to Laminin B2 gamma 1 - BSA and Azide free

Specificity

This antibody uniformly stains all human and murine basement membranes.

Tested applications

IP, IHC-P, IHC-Fr, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Murine EHS laminin preparation.

Positive control

LS174T cells. Normal colon or colon carcinoma.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 10mM PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

A5

Isotype

IgG2a

Light chain type

kappa

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix

  • Immunohistochemistry (Frozen sections) - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)Immunohistochemistry (Frozen sections) - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580) image (enlarge)

  • Immunoprecipitation - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)Immunoprecipitation - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab80580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 µg/ml.
IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml.
IP: Use at 2µg/mg of lysate. (Native and denatured)

Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes.

Cellular localization

Secreted, extracellular space.

Alternative names

  • LAMB 2 antibody
  • LAMB2 antibody
  • LAMC 1 antibody
  • LAMC1 antibody
  • Laminin B2 chain antibody
  • Laminin gamma 1 antibody
  • Laminin gamma 1 chain antibody
  • MGC87297 antibody
see all

Database links

Anti-Laminin B2 gamma 1 antibody [A5] - BSA and Azide free images:

  Immunohistochemistry (Frozen sections) - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)

Immunohistochemistry (Frozen sections) - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)

Frozen human tonsil stained with ab80580 at 2µg/ml using peroxidase-conjugate and DAB chromogen. Note basement membrane and cell surface staining of blood vessels.

  Immunoprecipitation - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)

Immunoprecipitation - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)

Native IP using LS174T cells:
IP 2µg/mg lysate.
WB at 2µg/ml.

References for Anti-Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)

This product has been referenced in:

  • Ljubimov AVet al. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest 72:461-73 (1995). ICC/IF, IHC-P, IHC-Fr, IP; Human, Mouse.Read more (PubMed: 7723285) »
  • Ljubimov AVet al. Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50:562-6 (1992). ICC/IF, IHC-P, IHC-Fr, IP; Human, Mouse.Read more (PubMed: 1371500) »

See all 3 publications for this product

Publishing research using ab80580? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"