Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)

Product Name 

Laminin B2 gamma 1 antibody [A5] - BSA and Azide free

Product type 

Primary antibodies

Description 

Rat monoclonal [A5] to Laminin B2 gamma 1 - BSA and Azide free

Immunogen 

Murine EHS laminin preparation.

Reacts with 
(species key)

Hu, Ms

Specificity 

This antibody uniformly stains all human and murine basement membranes.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, IP

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 µg/ml.
IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml.
IP: Use at 2µg/mg of lysate. (Native and denatured)

Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

LS174T cells. Normal colon or colon carcinoma.

Cellular localization 

Secreted, extracellular space.

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix

Relevance 

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

A5

Isotype 

IgG2a

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: 10mM PBS, pH 7.4

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)

  This product has been used in: (two most recent references)

Ljubimov AV et al. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest 72:461-73 (1995). ICC/IF, IHC-P, IHC-Fr, IP; Human, Mouse. PubMed: 7723285

Ljubimov AV et al. Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50:562-6 (1992). ICC/IF, IHC-P, IHC-Fr, IP; Human, Mouse. PubMed: 1371500

See all 3 publication references for this product.


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