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Immunohistochemistry (Frozen sections) - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)
![Immunohistochemistry (Frozen sections) - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)](/ps/datasheet/images/80/ab80580/Laminin-B2-gamma-1-Primary-antibodies-ab80580-1.jpg)
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Immunoprecipitation - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)
![Immunoprecipitation - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)](/ps/datasheet/images/80/ab80580/Laminin-B2-gamma-1-Primary-antibodies-ab80580-2.jpg)
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Product Name
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Laminin B2 gamma 1 antibody [A5] - BSA and Azide free
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See all Laminin B2 gamma 1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [A5] to Laminin B2 gamma 1 - BSA and Azide free
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Immunogen
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Murine EHS laminin preparation.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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This antibody uniformly stains all human and murine basement membranes.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC/IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 2 µg/ml. IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml. IP: Use at 2µg/mg of lysate. (Native and denatured)
Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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LS174T cells. Normal colon or colon carcinoma.
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Cellular localization
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Secreted, extracellular space.
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
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Relevance
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Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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A5
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None Constituents: 10mM PBS, pH 7.4
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Laminin B2 gamma 1 antibody [A5] - BSA and Azide free is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Laminin B2 gamma 1 antibody [A5] - BSA and Azide free images, references, products related to ab80580 and other tools.
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Laminin B2 gamma 1 antibody [A5] - BSA and Azide free images:
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Immunohistochemistry (Frozen sections) - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)
Frozen human tonsil stained with ab80580 at 2µg/ml using peroxidase-conjugate and DAB chromogen. Note basement membrane and cell surface staining of blood vessels.
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Immunoprecipitation - Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)
Native IP using LS174T cells:IP 2µg/mg lysate.WB at 2µg/ml.
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References for Laminin B2 gamma 1 antibody [A5] - BSA and Azide free (ab80580)
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This product has been used in: (two most recent references)
Ljubimov AV et al. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest 72:461-73 (1995). ICC/IF, IHC-P, IHC-Fr, IP; Human, Mouse. PubMed: 7723285
Ljubimov AV et al. Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50:562-6 (1992). ICC/IF, IHC-P, IHC-Fr, IP; Human, Mouse. PubMed: 1371500
See all 3 publication references for this product.
If you publish research using ab80580 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Laminin B2 gamma 1
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Laminin B2 gamma 1 antibody [A5] - BSA and Azide free - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"