Laminin B2 gamma 1 antibody [D18] - BSA and Azide free (ab80581)

Product Name 

Laminin B2 gamma 1 antibody [D18] - BSA and Azide free

Product type 

Primary antibodies

Description 

Mouse monoclonal [D18] to Laminin B2 gamma 1 - BSA and Azide free

Immunogen 

Rat glomerular basement membrane.

Reacts with 
(species key)

Hu, Rat, Gpig

Tested applications 
(see key)

IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 2 - 4 µg/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Ls174T cells, Skin.

Cellular localization 

Secreted, extracellular space.

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix

Relevance 

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

D18

Isotype 

IgG2a

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 10mM PBS, pH 7.4

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for Laminin B2 gamma 1 antibody [D18] - BSA and Azide free (ab80581)

  This clone has been used in: (two most recent references)

Green TL et al. Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells. J Biol Chem 267:2014-22 (1992). PubMed: 1730732

Sanes JR et al. Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol 111:1685-99 (1990). PubMed: 2211832
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