![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin B2 gamma 1 antibody [D18] - BSA and Azide free (ab80581) image](#Laminin-B2-gamma-1-Primary-antibodies-ab80581-1.jpg)
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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Overview
Product name
Anti-Laminin B2 gamma 1 antibody [D18] - BSA and Azide free
See all Laminin B2 gamma 1 products (4) ...
Description
Mouse monoclonal [D18] to Laminin B2 gamma 1 - BSA and Azide free
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Rat, Guinea pig, Human
Immunogen
Rat glomerular basement membrane.
Positive control
Ls174T cells, Skin.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
D18
Isotype
IgG2a
Light chain type
kappa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin B2 gamma 1 antibody [D18] - BSA and Azide free (ab80581)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin B2 gamma 1 antibody [D18] - BSA and Azide free (ab80581) image](/ps/datasheet/images/80/ab80581/Laminin-B2-gamma-1-Primary-antibodies-ab80581-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab80581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: Use at a concentration of 2 - 4 µg/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes.
Cellular localization
Secreted, extracellular space.
Alternative names
- LAMB 2 antibody
- LAMB2 antibody
- LAMC 1 antibody
see all
Database links
- Entrez Gene: 3915 Human
- Entrez Gene: 117036 Rat
- Omim: 150290 Human
- SwissProt: P11047 Human
- SwissProt: Q5VYE7 Human
- SwissProt: P97552 Rat
- Unigene: 609663 Human
- Unigene: 7145 Rat
Anti-Laminin B2 gamma 1 antibody [D18] - BSA and Azide free images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin B2 gamma 1 antibody [D18] - BSA and Azide free (ab80581)
Formalin-fixed, paraffin-embedded human skin stained with ab80581 at 4µg/ml using a peroxidase-conjugate and AEC chromogen. Note basement membrane staining of skin.
References for Anti-Laminin B2 gamma 1 antibody [D18] - BSA and Azide free (ab80581)
This product has been referenced in:
- Green TLet al. Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells. J Biol Chem 267:2014-22 (1992).Read more (PubMed: 1730732) »
- Sanes JRet al. Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol 111:1685-99 (1990).Read more (PubMed: 2211832) »
See all 2 publications for this product
Publishing research using ab80581? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"