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Overview

  • Product nameAnti-Laminin gamma 1 antibody [SPM277]See all Laminin gamma 1 primary antibodies ...
  • Description
    Rat monoclonal [SPM277] to Laminin gamma 1
  • Tested applicationsICC/IF, WB, IP, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Tissue / cell preparation. (Murine EHS laminin preparation)

  • Positive controlLS174T cells. Normal colon or colon carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab17792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use at an assay dependent dilution.
WB WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 210 kDa. The best results were obtained by incubating the primary antibody overnight at 4C.
IP IP: Use at 2 µg/mg of lysate.
IHC-P IHC-P: 1/50. Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000 U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37C.

Target

  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificityFound in the basement membranes (major component).
  • Sequence similaritiesContains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.

    • Target information above from: UniProt accession P11047 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      LAMB2 antibodyLAMC1 antibodyLAMC1_HUMAN antibody
      Laminin B2 chain antibodyLaminin subunit gamma-1 antibodyLaminin, gamma 1 (formerly LAMB2) antibodyLaminin-1 subunit gamma antibodyLaminin-10 subunit gamma antibodyLaminin-11 subunit gamma antibodyLaminin-2 subunit gamma antibodyLaminin-3 subunit gamma antibodyLaminin-4 subunit gamma antibodyLaminin-6 subunit gamma antibodyLaminin-7 subunit gamma antibodyLaminin-8 subunit gamma antibodyLaminin-9 subunit gamma antibodyMGC87297 antibodyS-LAM gamma antibodyS-laminin subunit gamma antibody
    see all

Anti-Laminin gamma 1 antibody [SPM277] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [SPM277] (ab17792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [SPM277] (ab17792)
    ab17792 at 1/50 staining Laminin gamma 1 from human tonsil by immunohistochemistry
  • Immunohistochemistry (Frozen sections) - Laminin gamma 1 antibody [SPM277] (ab17792)
    Immunohistochemistry (Frozen sections) - Laminin gamma 1 antibody [SPM277] (ab17792)Image courtesy of an anonymous Abreview.
    ab17792 staining Laminin gamma 1 in dog skeletal muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with acetone. Samples were incubated with the primary antibody at a 1/100 dilution for 8 hours at 4°C. An FITC-conjugated goat polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

References for Anti-Laminin gamma 1 antibody [SPM277] (ab17792)

This product has been referenced in:
  • Fournier B  et al. Blockade of the activin receptor IIb activates functional brown adipogenesis and thermogenesis by inducing mitochondrial oxidative metabolism. Mol Cell Biol 32:2871-9 (2012). Read more (PubMed: 22586266) »
  • Steenhard BM  et al. Transgenic Expression of Human LAMA5 Suppresses Murine Lama5 mRNA and Laminin a5 Protein Deposition. PLoS One 6:e23926 (2011). WB ; Mouse . Read more (PubMed: 21915268) »

See all 2 Publications for this product

Publishing research using ab17792 ? Please let us know so that we can cite the reference in this datasheet

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Immunohistochemistry (Frozen sections) abreview for Anti-Laminin gamma 1 antibody [SPM277]

 Excellent
Abreviews
Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative Acetone
Permeabilization No
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Submitted Oct 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"