Overview

  • Product nameLaminin Rat ELISA Kit
    See all Laminin kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s rat Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat Laminin in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Laminin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards (form rat sarcoma basement membrane) and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Laminin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat Laminin amount of sample captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Involvement in diseaseDefects in LAMB2 are the cause of Pierson syndrome (PIERSS) [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome.
    Defects in LAMB2 are a cause of congenital nephrotic syndrome (CONPHS) [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
  • Sequence similaritiesContains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.
  • Information by UniProt
  • Alternative names
    • Lamb2
    • LAMB2_HUMAN
    • LAMC1
    • LAMC2
    • LAMC3
    • laminin A
    • Laminin B
    • Laminin B1s chain
    • Laminin gamma 1
    • Laminin gamma 2
    • Laminin gamma 3
    • Laminin subunit beta-2
    • Laminin-11 subunit beta
    • Laminin-14 subunit beta
    • Laminin-15 subunit beta
    • Laminin-3 subunit beta
    • Laminin-4 subunit beta
    • Laminin-7 subunit beta
    • Laminin-9 subunit beta
    • OCCM
    • S-LAM beta
    • S-laminin subunit beta
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Laminin Rat ELISA Kit images

  • Representative Standard Curve using ab119573

Protocols

References for Laminin Rat ELISA Kit (ab119573)

ab119573 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119573.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"