Anti-Laminin alpha 5/LAMA5 antibody [4C7] (ab17107)
Key features and details
- Mouse monoclonal [4C7] to Laminin alpha 5/LAMA5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Laminin alpha 5/LAMA5 antibody [4C7]
See all Laminin alpha 5/LAMA5 primary antibodies -
Description
Mouse monoclonal [4C7] to Laminin alpha 5/LAMA5 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Full length native protein (purified) corresponding to Human Laminin alpha 5/LAMA5.
Database link: O15230 -
Positive control
- Skin
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General notes
This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment. -
Clonality
Monoclonal -
Clone number
4C7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
1/5 - 1/20. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/5 - 1/20. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver. -
Sequence similarities
Contains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer. -
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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Database links
- Entrez Gene: 3911 Human
- Omim: 601033 Human
- SwissProt: O15230 Human
- Unigene: 473256 Human
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Alternative names
- KIAA0533 antibody
- KIAA1907 antibody
- LAMA5 antibody
see all
Images
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ab17107 at 1/25 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image was generated using the ascites version of the product.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human renal carcinoma tissue, staining Laminin alpha 5/LAMA5 with ab17107.
This image was generated using the ascites version of the product.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab17107 has been referenced in 2 publications.
- Ke FY et al. Novel monoclonal antibody against integrin a3 shows therapeutic potential for ovarian cancer. Cancer Sci 111:3478-3492 (2020). PubMed: 32648337
- Mackey AL et al. Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle. FASEB J 25:1943-59 (2011). IHC-Fr ; Human . PubMed: 21368102