Anti-Laminin antibody (ab11575)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin antibody (ab11575)

This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.

  Immunohistochemistry (Frozen sections) - Laminin antibody (ab11575)

ab11575 at a 1/200 dilution staining mouse placenta tissue sections by Immunohistochemistry (frozen sections). The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated goat anti-rabbit IgG.

This image is courtesy of an anonymous Abreview

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin antibody (ab11575)

ab11575 staining Laminin in mouse oocytes by Immunohistochemistry (PFA fixed, paraffin embedded sections).

Grafts were fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned in 5 to 8 µm intervals. In brief, sections on slides were de-paraffinized, re-hydrated, antigens unmasked by incubating in target retrieval solution at 95°C for 30 minutes, permeabilized in 0.1% Triton-X100 for 5 minutes, blocked with 10% chicken serum in TBST overnight, and incubated with primary antibody at 1/200, in TBST with 1% serum for 1 hour at room temperature. After washing in TBST, slides were incubated with secondary antibody at 1/1000, for 30 minutes at room temperature. Cover slips were mounted with Prolong Gold Antifade with DAPI.

Immunofluorescence revealed TRA98+ oocytes and FOXL2+ granulosa cells in ovarian cord-like structures (dashed lines) and Laminin+ basement membrane (red) following five days of intact e12.5 female genital ridge transplantation. Along with disrupted ovarian cord formation, re-aggregation also resulted in a uniform distribution of PECAM+ and Laminin+ cells amongst dispersed oocytes and granulosa cells that correlated with subsequent oocyte loss. 100× magnification.

Image from Dr CR Nicholas et al, BMC Dev Biol. 2010 Jan 8;10:2, Fig 6.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin antibody (ab11575)

ab11575 staining Laminin in Mouse skin neoplasia - papilloma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/200) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Pawel Mazur

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  Immunohistochemistry (Frozen sections) - Laminin antibody (ab11575)

ab11575 staining Laminin in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with PBST (Triton X 100 0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/250) for 1 hour. An undiluted Alexa Fluor^®488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. The image shows 2 differeent skin sections with Laminin (green) staining in the basal layer of the epidermis. DAPI staining in blue.

This image is courtesy of an Abreview submitted by Pawel Mazur

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  Immunocytochemistry/ Immunofluorescence - Laminin antibody (ab11575)

ab11575 staining Laminin in decellularized lung extracellular matrix by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde and blocked with 2% serum for 2 hours at 25°C. Samples were then incubated with ab11575 at a 1/100 dilution for 20 minutes at 4°C. The secondary used was an FITC conjugated goat anti-rabbit IgG used at a 1/100 dilution.

Image courtesy of an anonymous Abreview.

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