Overview

  • Product nameAnti-Laminin antibody
    See all Laminin primary antibodies
  • Description
    Rabbit polyclonal to Laminin
  • SpecificityLaminin fragment P1 100%, collagen type I and IV <0.1%, fibronectin <0.1%
  • Tested applicationsRIA, Sandwich ELISA, ICC/IF, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified laminin P1 fragment from human placenta.

  • Positive control
    • Human skin
  • General notes


    This antibody primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion.

Properties

Applications

Our Abpromise guarantee covers the use of ab23753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA 1/1000.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [LAM-89] to Laminin (ab49726). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab49726 as Detection.
ICC/IF Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificityBroadly expressed in: skin, heart, lung, and the reproductive tracts.
  • Sequence similaritiesContains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMA 1 antibody
    • LAMA antibody
    • LAMA1 antibody
    • LAMB1 antibody
    • Lamc3 antibody
    • LAMC3_HUMAN antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 1 chain antibody
    • Laminin B1 chain antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit gamma-3 antibody
    • Laminin-12 subunit gamma antibody
    • Laminin-14 subunit gamma antibody
    • Laminin-15 subunit gamma antibody
    see all

Anti-Laminin antibody images

Protocols

References for Anti-Laminin antibody (ab23753)

ab23753 has not yet been referenced specifically in any publications.

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