Overview
- Product nameAnti-Laminin antibodySee all Laminin primary antibodies ...
- DescriptionRabbit polyclonal to Laminin
- SpecificityLaminin fragment P1 100%, collagen type I and IV <0.1%, fibronectin <0.1%
- Tested applicationsRIA, Sandwich ELISA, ICC/IF, IHC-P, ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Purified laminin P1 fragment from human placenta.
- Positive controlHuman skin
- General notes
This antibody primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Concentration information loading... - PurityPreadsorbed IgG fraction
- Purification notesIgG fraction from ionen exchange chromatography
- Primary antibody notes This antibody primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab23753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| RIA | RIA: 1/1000. |
| Sandwich ELISA | sELISA: Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [LAM-89] to Laminin (ab49726). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab49726 as Detection. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
- Tissue specificityBroadly expressed in: skin, heart, lung, and the reproductive tracts.
- Sequence similaritiesContains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. - DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain IV is globular. - Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
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Database links
- Entrez Gene: 284217 Human
- Entrez Gene: 3908 Human
- Entrez Gene: 3912 Human
- Entrez Gene: 3913 Human
- Entrez Gene: 3918 Human
- Entrez Gene: 3915 Human
- Entrez Gene: 10319 Human
- Omim: 150320 Human
- SwissProt: P25391 Human
- SwissProt: P24043 Human
- SwissProt: P07942 Human
- SwissProt: P55268 Human
- SwissProt: Q9Y6N6 Human
- Unigene: 270364 Human
- Unigene: 201805 Human
see all
Target information above from: UniProt accession
Q9Y6N6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- LAMA 1 antibodyLAMA antibodyLAMA1 antibody
- LAMB1 antibodyLamc3 antibodyLAMC3_HUMAN antibodyLaminin A chain antibodyLaminin alpha 1 antibodyLaminin alpha 1 chain antibodyLaminin B1 chain antibodyLaminin subunit beta 1 antibodyLaminin subunit gamma-3 antibodyLaminin-12 subunit gamma antibodyLaminin-14 subunit gamma antibodyLaminin-15 subunit gamma antibody
see all
Anti-Laminin antibody images
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Sandwich ELISA - Laminin antibody (ab23753)Standard curve for Laminin; dilution range 1pg/ml to 1µg/ml using Capture Antibody Rabbit polyclonal to Laminin (ab23753) at 5µg/ml and Detector Antibody Mouse monoclonal [LAM-89] to Laminin (ab49726) at 0.5µg/ml.
Protocols
References for Anti-Laminin antibody (ab23753)
ab23753 has not yet been referenced specifically in any publications.
