Anti-Laminin beta 1 antibody (ab69633)

Overview

• Product nameAnti-Laminin beta 1 antibodySee all Laminin beta 1 primary antibodies ...
• Description
  Rabbit polyclonal to Laminin beta 1
• Tested applicationsWB, ELISA, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthesized peptide derived from the C-terminus of human Laminin beta 1.

• Positive controlHela cells, human liver carcinoma tissue and extracts from HepG2

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Basal Lamina

  • Cardiovascular
  • Angiogenesis
  • Adhesion / ECM
  • Extracellular Matrix

Applications

Target

• FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
• Sequence similaritiesContains 13 laminin EGF-like domains.
  Contains 1 laminin IV type B domain.
  Contains 1 laminin N-terminal domain.
• DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
  Domains VI and IV are globular.
• Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Major component.

Target information above from: UniProt accession P07942 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3912 Human
  • Entrez Gene: 16777 Mouse
  • Omim: 150240 Human
  • SwissProt: P07942 Human
  • SwissProt: P02469 Mouse
  • Unigene: 650585 Human
  • Unigene: 172674 Mouse

• Alternative names
  CLM antibodyCutis laxa with marfanoid phenotype antibodyLAM B1 antibody

  LAMB 1 antibodyLAMB1 antibodyLAMB1_HUMAN antibodyLaminin B1 antibodyLaminin B1 chain antibodyLaminin beta 1 chain antibodyLaminin beta 1 chain precursor antibodyLaminin beta1 antibodyLaminin subunit beta 1 antibodyLaminin subunit beta-1 antibodyLaminin-1 subunit beta antibodyLaminin-10 subunit beta antibodyLaminin-12 subunit beta antibodyLaminin-2 subunit beta antibodyLaminin-6 subunit beta antibodyLaminin-8 subunit beta antibodyMGC142015 antibody

  see all

Anti-Laminin beta 1 antibody images

• 

  Western blot - Laminin beta 1 antibody (ab69633)
  All lanes : Anti-Laminin beta 1 antibody (ab69633) at 1/500 dilution
  
  Lane 1 : Extracts from HepG2 cells
  Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 198 kDa
  Observed band size : >170 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 110 kDa,130 kDa,60 kDa,67 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin beta 1 antibody (ab69633)
  Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab69633 at 1:50 dilution.
  Left image un-treated.
  Right image treated with immunizing peptide.
• 

  Immunocytochemistry/ Immunofluorescence - Laminin beta 1 antibody (ab69633)
  Immunofluorescence analysis of HeLa cells using ab69633 at a 1:500 dilution.
  Left image un-treated.
  Right image treated with immunizing peptide.