Anti-Laminin gamma 1 antibody [BC17] (ab41823)
Key features and details
- Mouse monoclonal [BC17] to Laminin gamma 1
- Suitable for: ELISA, IHC-Fr, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Laminin gamma 1 antibody [BC17]
See all Laminin gamma 1 primary antibodies -
Description
Mouse monoclonal [BC17] to Laminin gamma 1 -
Host species
Mouse -
Specificity
The antibody recognizes the gamma 1 chain of human reduced and alkylated laminin produced by various cultured cells, which produce laminins complexed with it. The specificity of clone BC17 has been confirmed by immunoprecipitation and ELISA by using recombinant Laminin gamma 1. -
Tested applications
Suitable for: ELISA, IHC-Fr, IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Pepsin-digested laminin from human placenta.
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General notes
Balb/c spleen cells used to produce hybridoma.The monoclonal antibody BC17 against Laminin gamma 1 can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing this chain. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning e.g. the integrity of the basement membranes in carcinomas.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
The monoclonal antibody BC17 against Laminin gamma 1 can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing this chain. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning e.g. the integrity of the basement membranes in carcinomas. -
Clonality
Monoclonal -
Clone number
BC17 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Found in the basement membranes (major component). -
Sequence similarities
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 3915 Human
- Omim: 150290 Human
- SwissProt: P11047 Human
- SwissProt: Q5VYE7 Human
- SwissProt: Q6NVY8 Human
- Unigene: 609663 Human
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Alternative names
- LAMB2 antibody
- lamc1 antibody
- LAMC1_HUMAN antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab41823 has been referenced in 1 publication.
- Geberhiwot T et al. Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin beta1 and gamma1 chains. Matrix Biol 19:163-7 (2000). PubMed: 10842099