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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Overview
Product name
Anti-Laminin gamma 1 antibody [D18]
See all Laminin gamma 1 products (6) ...
Description
Mouse monoclonal [D18] to Laminin gamma 1
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Guinea pig
Immunogen
Rat glomerular basement membrane.
Positive control
LS174T cells. Skin tissue sections.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin / nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Clonality
Monoclonal
Clone number
D18
Isotype
IgG2a
Light chain type
kappa
Research areas
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Application notes
Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4µg / ml for 30 min at RT.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
For staining of formalin fixed tissues, digest sections with Protease XXV at 1mg / ml PBS for 5 minutes at 37°C.
Target
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificity
Found in the basement membranes (major component).
Sequence similarities
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane.
Target information above from: UniProt accessionP11047
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LAMB2 antibody
- LAMC1 antibody
- LAMC1_HUMAN antibody
see all
Database links
- Entrez Gene: 3915 Human
- Entrez Gene: 117036 Rat
- Omim: 150290 Human
- SwissProt: P11047 Human
- SwissProt: Q5VYE7 Human
- SwissProt: Q6NVY8 Human
- Unigene: 609663 Human
- Unigene: 7145 Rat
References for Anti-Laminin gamma 1 antibody [D18] (ab3297)
ab3297 has not yet been referenced specifically in any publications.
Publishing research using ab3297? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"