Anti-Laminin gamma 1 antibody (ab69632)
Key features and details
- Rabbit polyclonal to Laminin gamma 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Laminin gamma 1 antibody
See all Laminin gamma 1 primary antibodies -
Description
Rabbit polyclonal to Laminin gamma 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthesized peptide derived from the internal region of human Laminin gamma 1.
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Positive control
- Extracts from HUVEC cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately >170 kDa (predicted molecular weight: 178 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately >170 kDa (predicted molecular weight: 178 kDa). |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Found in the basement membranes (major component). -
Sequence similarities
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 3915 Human
- Entrez Gene: 226519 Mouse
- Entrez Gene: 117036 Rat
- Omim: 150290 Human
- SwissProt: P11047 Human
- SwissProt: Q5VYE7 Human
- SwissProt: Q6NVY8 Human
- SwissProt: P02468 Mouse
see all -
Alternative names
- LAMB2 antibody
- lamc1 antibody
- LAMC1_HUMAN antibody
see all
Images
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All lanes : Anti-Laminin gamma 1 antibody (ab69632) at 1/500 dilution
Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 178 kDa
Observed band size: >170 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab69632 has been referenced in 5 publications.
- Sun L et al. The novel mutation P36R in LRP5L contributes to congenital membranous cataract via inhibition of laminin ?1 and c-MAF. Graefes Arch Clin Exp Ophthalmol 258:2737-2751 (2020). PubMed: 32789677
- Yan Y et al. Laminin a4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract. Aging (Albany NY) 11:2699-2723 (2019). PubMed: 31076560
- Yan Y et al. Laminins in an in vitro anterior lens capsule model established using HLE B-3 cells. Mol Med Rep 17:5726-5733 (2018). PubMed: 29436687
- Wei X et al. Kojic acid inhibits senescence of human corneal endothelial cells via NF-?B and p21 signaling pathways. Exp Eye Res 180:174-183 (2018). PubMed: 30597146
- Little GH et al. Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion. Nucleic Acids Res 40:3538-47 (2012). WB ; Human . PubMed: 22187159