General notesTissue: Larynx
Diagnosis: Squamous cell carcinoma, well differentiated
Stage: III T4N0MX
Age: 71 years
Gross findings: Tumor size 2cm, color red/ brown, friable. Cut surface heterogenous. Invaded.
Soluble protein fraction
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
Lysate notesThis product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction.
Please find below a range of products that relate to ab44736:
Extraction 1: soluble protein fraction
ab44736 Human Larynx Tumor Tissue lysate
ab44735 Human Larynx Normal Tissue lysate (matched)
Extraction 2: insoluble protein fraction
ab44738 Human Larynx Tumor Tissue lysate
ab44737 Human Larynx Normal Tissue lysate (matched)