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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Anti-Lbx2 antibody
See all Lbx2 products (3) ...
Mouse monoclonal to Lbx2
ELISA, ICC/IF, WBmore details
Reacts with
Human
Predicted to work with
Cow, Chimpanzee, Rhesus monkey
Recombinant fragment: ELERRFVFQK YLAPSERDGL ATRLGLANAQ VVTWFQNRRA KLKRDVEEMR ADVASLRALS PEVLCSLALP EGAPDPGLCL GPAGPDSRPH LSDEEIQVDD, corresponding to amino acids 95-195 of Human Lbx2 (NP_001009812) with tag.
ELERRFVFQK YLAPSERDGL ATRLGLANAQ VVTWFQNRRA KLKRDVEEMR ADVASLRALS PEVLCSLALP EGAPDPGLCL GPAGPDSRPH LSDEEIQVDD
Hela cells. Recombinant tagged human Lbx2 fragment.
Liquid
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein A purified
Monoclonal
IgG2a
kappa
Our Abpromise guarantee covers the use of ab76728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 10 µg/ml
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 21 kDa.
Lbx2 contains one homeobox DNA-binding domain and is a putative transcription factor. There are two named isoforms.
Nuclear
Western blot - Lbx2 antibody (ab76728)

Anti-Lbx2 antibody (ab76728) at 1 µg/ml + Recombinant tagged human Lbx2 fragment at 0.1 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 21 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
Immunocytochemistry/ Immunofluorescence - Lbx2 antibody (ab76728)

ab76728, at 10µg/ml, staining Lbx2 in Hela cells by immunofluorescence.
ab76728 has not yet been referenced specifically in any publications.
Publishing research using ab76728? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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Anti-Lbx2 antibody (ab76728) at 1 µg/ml + Recombinant tagged human Lbx2 fragment at 0.1 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 21 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.

ab76728, at 10µg/ml, staining Lbx2 in Hela cells by immunofluorescence.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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