Lbx2 antibody (ab78742)

Product Name 

Lbx2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Lbx2

Immunogen 

Synthetic peptide: ATPQPSEGRAAPEAPPGP, corresponding to amino acids 60-77 of Mouse Lbx2
BLAST 'ATPQPSEGRAAPEAPPGP' with or

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

IHC-FoFr, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-Fr: 1/200 - 1/1000. Fix with cold Acetone/Methanol (50/50) 2 min.
IHC-FoFr: 1/200 - 1/1000.
WB: 1/200 - 1/1000. Predicted molecular weight: 21 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other

Relevance 

Lbx2 contains one homeobox DNA-binding domain and is a putative transcription factor. There are two named isoforms.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 50% Ammonium sulphate, PBS

Form 

Lyophilised

Storage instructions 

Store at -70°C

Notes 

PREPARATION AND USE:
To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 µL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.
Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL.
Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solution can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Customer reviews (feedback) regarding Lbx2 antibody

Customer FAQs regarding Lbx2 antibody

Protocols for Lbx2 antibody

Price and availability of products related to Lbx2 antibody

Lbx2 antibody - related products: