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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-Lck antibody
See all Lck products (18) ...
Description
Rabbit polyclonal to Lck
Tested applications
IHC-P, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) corresponding to the C-terminus of human Lck.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Secreted Molecules >> Other secreted molecules
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Immunology >> Immunoglobulins >> Receptors
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/ml
WB: Use a concentration of 0.5 - 4 µg/ml.Predicted molecular weight: 58 kDa.Can be blocked with Lck peptide (ab52217).
IP: Use a concentration of 20 - 25 µg/ml.
Target
Function
Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.
Tissue specificity
Expressed specifically in lymphoid cells.
Involvement in disease
Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Domain
The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.
Post-translational
modifications
Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
Cellular localization
Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
Target information above from: UniProt accessionP06239
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LCK antibody
- Lck p56 antibody
- LCK_HUMAN antibody
see all
Database links
- Entrez Gene: 3932 Human
- Entrez Gene: 16818 Mouse
- Entrez Gene: 313050 Rat
- GenBank: NM_005356 Human
- GenBank: NM_010693 Mouse
- Omim: 153390 Human
- SwissProt: P06239 Human
- SwissProt: P06240 Mouse
see all
Anti-Lck antibody images:
Western blot - Anti-Lck antibody (ab18896)
Anti-Lck antibody (ab18896) at 1 µg/ml + Lck protein (Active) (ab79626) at 0.01 µg/ml
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody (ab18896)
IHC image of ab18896 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18896, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Lck antibody (ab18896)
This product has been referenced in:
- Shi Yet al. Regulatory T cells protect mice against coxsackievirus-induced myocarditis through the transforming growth factor beta-coxsackie-adenovirus receptor pathway. Circulation 121:2624-34 (2010).Read more (PubMed: 20530002) »
- Strasner ABet al. The Src kinase Lck facilitates assembly of HIV-1 at the plasma membrane. J Immunol 181:3706-13 (2008). WB; Human.Read more (PubMed: 18714047) »
See all 2 publications for this product
Publishing research using ab18896? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"