Anti-Lck antibody (ab3885)

Western blot - Lck antibody (ab3885)

All lanes : Anti-Lck antibody (ab3885) at 1/1000 dilution

Lane 1 : COS cells transfected with mouse Fyn
Lane 2 : COS cells transfected with mouse Lck


Predicted band size : 57 kDa

Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody (ab3885)

Confocal immunofluorescence image of a COS cell transfected with a mouse Lck construct and stained with ab3885 at 1/200.

In untransfected cells, some nuclear staining is also visible as well as Lck staining.

Western blot - Lck antibody (ab3885)

All lanes : Anti-Lck antibody (ab3885) at 0.87 µg/ml

Lane 1 : HeLa nuclear extract
Lane 2 : HeLa whole cell extract
Lane 3 : A431 cell extract
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HeLa nuclear extract with Lck peptide (ab13750) at 1 µg/ml
Lane 7 : HeLa whole cell extract with Lck peptide (ab13750) at 1 µg/ml
Lane 8 : A431 cell extract with Lck peptide (ab13750) at 1 µg/ml
Lane 9 : Jurkat cell lysate with Lck peptide (ab13750) at 1 µg/ml
Lane 10 : HEK293 cell lysate with Lck peptide (ab13750) at 1 µg/ml


Predicted band size : 57 kDa
Observed band size : 57 kDa
Additional bands at : 25 kDa (possible non-specific binding),35 kDa (possible non-specific binding).

ab3885 recognises a band at approximately 57 kDa, corresponding to Lck in HeLa nuclear (lane1), HeLa whole cell (lane2) and Jurkat cell (lane4) lysates. The recognition of these bands is succesfully blocked using the immunizing peptide (lanes 6, 7, 9 respectively). Lck is not detected using ab3885 in A431 (lane3) or HEK293 (lane5) cells under these conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lck antibody (ab3885)

ab3885 staining Lck in human thymus tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval and blocking was done for 15 minutes at 20°C (5 minutes of peroxidase block then 10 minutes of protein block) . The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used, undiluted as secondary.

This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.