Lck antibody (ab3885)

Product Name 

Lck antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Lck

Immunogen 

Synthetic peptide derived from residues 200 - 300 of Human Lck.

Reacts with 

Ms, AGMk

Predicted to react 
(species key)

Hu, Chk
(Due to sequence homology)

Specificity 

This antibody was designed to recognise the activated form of Lck and was raised for that purpose against the linker that connects the SH2 domain and the tyrosine kinase domain of the kinase. This linker sits in the SH3 substrate binding site in the inactive kinase. The position of the linker is based on the crystal structure of inactive Src.

However, the antibody does not seem to distinguish the active and inactive forms of Lck. In IF, it recognises kinase dead Lck at the same concentration as constitutively active Lck.

In western blot, the antibody is highly specific for Lck versus other tyrosine kinases (see blot). Even, if the antibody was specific for active Lck in IF , it would be expected to recognise both active and inactive forms in Western blot. This is because the linker that the antibody recognises is present in both active and inactive Lck, it was just expected to be concealed in native form inactive Lck.

Tested applications 
(see key)

ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IF: 1/200 - 1/500.
IHC-P: 1/2000 (See abreview).
WB: 1/1000. Predicted molecular weight: 57 kDa. Can be blocked with Lck peptide (ab13750) or Lck peptide (ab13750).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Bound to the cytoplasmic domain of either CD4 or CD8.

Research areas 

Immunology >> Secreted Molecules >> Other secreted molecules
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Immunology >> Immunoglobulins >> Receptors
Immunology >> Adaptive Immunity >> T Cells >> CD

Relevance 

Lck is a lymphoid specific cytosolic protein tyrosine kinase (PTK), which is essential for T cell development and function. It is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T cell antigen receptor (TCR) linked signal transduction pathways. Association of the TCR with a peptide antigen bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine based activation motifs (ITAMs) in the cytoplasmic tails of the TCR chains and CD3 subunits. The phosphoITAMs serve as docking sites for Src homology domain 2 (SH2) containing molecules, predominantly ZAP 70 and Syk. Only then can ZAP 70 undergo tyrosine phosphorylation, become enzymatically active and further phosphorylate downstream effector molecules. In addition, Lck contributes to signaling by other receptor molecules. Lck is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature TCR.

Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.874 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Lck antibody images, references, products related to ab3885 and other tools.

Lck antibody images:

References for Lck antibody (ab3885)

  This product has been used in:

Rder C et al. The tumor-associated antigen EBAG9 negatively regulates the cytolytic capacity of mouse CD8+ T cells. J Clin Invest 119:2184-203 (2009). IHC (PFA fixed); Mouse. PubMed: 19620783

If you publish research using ab3885 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Lck antibody

Customer FAQs regarding Lck antibody

Protocols for Lck antibody

Price and availability of products related to Lck antibody

Lck antibody - related products: