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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - Anti-Lck (phospho Y505) antibody (ab4901)
Predicted band size : 60 kDa
Peptide Competition: Full length untagged recombinant human Lck protein was added to control cell extract which does not contain Lck and then resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins then were transferred to nitrocellulose and incubated with 0.50 µg/mL ab4901 antibody, following prior incubation with: (1) the appropriately phosphorylated peptide immunogen, (2) the nonphosphorylated peptide corresponding to the peptide immunogen, (3) the non-phosphorylated peptide derived from the corresponding region of Src, (4) a generic phosphotyrosine containing peptide, (5) no peptide, and (6) the phosphorylated peptide derived from the corresponding region of Src. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStar detection method. The data presented here demonstrate that only the phosphopeptide corresponding to this site completely blocks the antibody signal, demonstrating the specificity of ab4901 antibody for this phosphorylated residue.
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