Anti-Legionella pneumophila hsp60 antibody [18] (ab81137)

Overview

  • Product nameAnti-Legionella pneumophila hsp60 antibody [18]
  • Description
    Mouse monoclonal [18] to Legionella pneumophila hsp60
  • Specificityab81137 recognizes recombinant Legionella pneumophila hsp60 protein.
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with Recombinant Fragment. Predicted to react with
  • Immunogen

    Recombinant fragment, corresponding to amino acids 272 - 478 of Legionella pneumophila hsp60 protein (YP_094724), expressed in E.coli

  • Positive control
    • Recombinant Legionella pneumophila hsp60 antigen.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Proclin
    Constituents: Tris HCl, Sodium citrate, pH 7.0
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • Clone number18
  • IsotypeIgG2a
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab81137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 58 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceLegionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaires' disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). Hsp60 is a member of the GroEL family of molecular chaperones and may exist in extracytoplasmic locations of Legionella pneumophila.
    • Cellular localizationCytoplasmic
    • Alternative names
      • 58 kDa common antigen antibody
      • 60 kDa chaperonin antibody
      • groEL protein antibody
      • groL antibody
      • Heat shock protein B antibody
      • hsp60 antibody
      see all

    Anti-Legionella pneumophila hsp60 antibody [18] images

    • All lanes : Anti-Legionella pneumophila hsp60 antibody [18] (ab81137) at 1/1000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Recombinant Legionella pneumophila hsp60 antigen


      Predicted band size : 58 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Legionella pneumophila hsp60 antibody [18] (ab81137)

    ab81137 has not yet been referenced specifically in any publications.

    Product Wall

    Vielen Dank für Ihre Anfrage. In der Tat haben wir einige polyklonalen HSP60- Antikörper in unserem Katalog verfügbar, unter anderem zum Beispiel ab105853: ein Kaninchen-polyklonaler, der gegen das humane Volllängenprotein gerichtet ist und für West...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"