Overview

  • Product nameAnti-Legumain antibodySee all Legumain primary antibodies ...
  • Description
    Rabbit polyclonal to Legumain
  • Tested applicationsWB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 100-190 of Human Legumain. This sequence is identical in Mouse and Rat.

  • Positive control
    • HT29, HeLa, and Mouse Kidney lysates; Human ovary carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab125286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionHas a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
  • Tissue specificityUbiquitous. Particularly abundant in kidney, heart and placenta.
  • Sequence similaritiesBelongs to the peptidase C13 family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationLysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • AEP antibody
    • Asparaginyl endopeptidase antibody
    • cysteine 1 antibody
    • Cysteine protease 1 antibody
    • EC 3.4.22.34 antibody
    • Legumain antibody
    • LGMN antibody
    • LGMN_HUMAN antibody
    • LGMN1 antibody
    • Protease antibody
    • Protease cysteine 1 (legumain) antibody
    • Protease cysteine 1 antibody
    • PRSC1 antibody
    see all

Anti-Legumain antibody images

  • All lanes : Anti-Legumain antibody (ab125286) at 1/500 dilution

    Lane 1 : HT29 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Mouse Kidney lysate


    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab125286, at 1/200 dilution, staining Legumain in formalin-fixed, paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

References for Anti-Legumain antibody (ab125286)

ab125286 has not yet been referenced specifically in any publications.

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