Anti-Leishmania A2 antibody [C9] (ab150344)
Key features and details
- Mouse monoclonal [C9] to Leishmania A2
- Suitable for: WB, IP, ICC/IF
- Reacts with: Leishmania
- Isotype: IgG1
Overview
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Product name
Anti-Leishmania A2 antibody [C9] -
Description
Mouse monoclonal [C9] to Leishmania A2 -
Host species
Mouse -
Specificity
A sensitive antibody to Leishmania amastigote-specific A2 protein. This sequence is particular to the parasite and therefore the antibody has no cross-reactivity with mammalian proteins. It is specific to A2 protein from Leishmania donovani and in a highly probable manner to other species which express A2. It won't be detected in species where the A2 is a non-expressed pseudogene such as Leishmania major.
ab150344 recognizes the family of A2 proteins ranging from 42 to 100kDa.
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Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Leishmania -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 99% PBS
Contains no additives. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Lyophilized protein G purified in PBS pH7.4 -
Clonality
Monoclonal -
Clone number
C9 -
Isotype
IgG1
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100.
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IP |
Use at an assay dependent dilution.
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ICC/IF |
Use at an assay dependent dilution.
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Notes |
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WB
1/100. |
IP
Use at an assay dependent dilution. |
ICC/IF
Use at an assay dependent dilution. |
Target
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Relevance
Leishmania donovani protozoa is responsible for human visceral leishmaniasis. During their life cycle, the parasites exist in 2 forms: as flagellated promastigote within the sandfly vector and as non flagellated amastigote in the macrophage phagolysosomal compartment of the mammalian host. A2 protein is an amastigote specific virulence factor that is required for Leishmania donovani parasite survival in a mammalian host.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab150344 has been referenced in 3 publications.
- Papadaki A et al. The Leishmania donovani LDBPK_220120.1 Gene Encodes for an Atypical Dual Specificity Lipid-Like Phosphatase Expressed in Promastigotes and Amastigotes; Substrate Specificity, Intracellular Localizations, and Putative Role(s). Front Cell Infect Microbiol 11:591868 (2021). PubMed: 33842381
- Giri S & Shaha C Leishmania donovani parasite requires Atg8 protein for infectivity and survival under stress. Cell Death Dis 10:808 (2019). PubMed: 31649242
- Domínguez-Bernal G et al. HisAK70: progress towards a vaccine against different forms of leishmaniosis. Parasit Vectors 8:629 (2015). PubMed: 26653170