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Overview

  • Product nameLeptin Human ELISA KitSee all Leptin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 2 pg/ml
  • Range
    1.64 pg/ml - 400 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.42 84% - 104%
    Serum 94.49 83% - 103%
    Plasma 93.85 82% - 102%

  • Species reactivity
    Reacts with: Human
  • Product overview

    Leptin is a secreted protein of 16 kDa. Human and murine leptin show approximately 84 percent identity at the protein level. Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Studies indicate that Human obesity may be associated with leptin receptor deficiencies rather than constituting a problem with leptin itself.

    ab100581 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Leptin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml
    Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody Leptin: biotinylated anti-human Leptin 2 vials
    HRP-Streptavidin Concentrate 160X Item G 1 x 200µl
    Leptin Human Standard(recombinant) 2 vials
    Leptin Microplate (12 strips x 8 wells) coated with anti-human Leptin 1 unit
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P41159 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        FLJ94114LEPLEP_HUMAN
        LeptinLeptin Murine Obesity Homolog Leptin Precursor Obesity FactorOBObese proteinObesity factorObesity homolog mouse Obesity murine homolog leptinOBS
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100581 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Leptin Human ELISA Kit images

      • ELISA - Leptin Human ELISA Kit (ab100581)
        ELISA - Leptin Human ELISA Kit (ab100581)

      Protocols

      References for Leptin Human ELISA Kit (ab100581)

      ab100581 has not yet been referenced specifically in any publications.

      Publishing research using ab100581 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100581 Submit a Question for ab100581

      Displaying 1 - 6 of 6 results for Abreviews and Q&A

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      What is the difference between products: ab 108879 and ab100581?

      These products are very similar, they use similar techniques to measure human Leptin in samples. In reviewing the information about each kit I have noted that ab100581 Sensitivity< 2 pg/ml, Range of 1.64 pg/ml - 400 pg/ml and Recovery of 94%. While ab1...

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      Inquiry: I am interested in working with the leptin ELISA kit for a human population. My question is: how well does the kit work for urine sample in adolescent humans? Is it necesary to concentrate the sample in order to measure the hormone? Tha...

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      Thank you for contacting us. We do not specifically have data on adolescents but can tell you that typically human urine does not have to be concentrated and can be diluted using the kit if necessary.

      I hope this information is helpful to you. P...

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      Good Afternoon.
      We would like to ask for the quoting of the following products:
      Leptin Human ELISA Kit (ab100581)
      LeptinR Human ELISA Kit (ab119574)
      Our website refers that both kits have the same price, though the kit
      ab100581 has mo...

      Read More

      Thank you for contacting us.

      The differences of the two kits you are interested in are listed below. The most important difference is that one of the Kits is for detection of the Leptin Receptor, while the other kit is only detecting the secret...

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      I would like to measure Leptin in either plasma or serum of pigs. I would like to know the chances of one of the abcam available leptin ELISA kits to work in pigs. Find below the pig leptin aminoacid sequence
      MRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTIVTR...

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      Thank you very much for your inquiry and your interest in our kits.

      Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

      For the other three kits, ab100581, ab100718 and ab100773,the laboratory...

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      i read about your Leptin Human ELISA Kit - 1 x 96 Well Plate (ab100581) it´s verry interesting i have a question That kit would be useful in rabbit serum? thank you for you answer

      Thank you for contacting Abcam. Unfortunately, we do not have any cross reactivity data for rabbit samples on the ab100581 kit. Therefore we do not know if the kit will work with rabbit serum samples. Both the capture and detection antibody are monoclonal...

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      Dear sir/mdm,   I would like to check if the Leptin standard protein in the kit ab100581 is sold separately. If yes, please advise on the catalog number. Thank you.   Regards,    

      Thank you for your enquriy. I am sorry to confirm that the Leptin standard protein from kit ab100581 is not sold separately. If you have any further questions, please do not hesitate to contact us.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"