Leptin Human ELISA Kit (ab100581)
- Product nameLeptin Human ELISA KitSee all Leptin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range1.64 pg/ml - 400 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.42 84% - 104% Serum 94.49 83% - 103% Plasma 93.85 82% - 102%
- Species reactivityReacts with: Human
- Product overview
Leptin is a secreted protein of 16 kDa. Human and murine leptin show approximately 84 percent identity at the protein level. Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Studies indicate that Human obesity may be associated with leptin receptor deficiencies rather than constituting a problem with leptin itself.
ab100581 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Leptin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody Leptin: biotinylated anti-human Leptin 2 vials HRP-Streptavidin Concentrate 160X Item G 1 x 200µl Leptin Human Standard(recombinant) 2 vials Leptin Microplate (12 strips x 8 wells) coated with anti-human Leptin 1 unit Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- LeptinLeptin Murine Obesity Homolog Leptin Precursor Obesity FactorOBObese proteinObesity factorObesity homolog mouse Obesity murine homolog leptinOBS
Our Abpromise guarantee covers the use of ab100581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Leptin Human ELISA Kit (ab100581)
ab100581 has not yet been referenced specifically in any publications.