Leptin Human ELISA Kit (ab108879)
- Product nameLeptin Human ELISA KitSee all Leptin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.12 ng/ml
- Range0.125 ng/ml - 32 ng/ml
= 99.5 %
- Assay time5h 00m
- Species reactivityReacts with: Human
- Product overview
Leptin, a 16 kDa protein secreted from white adipocytes, has been implicated in the regulation of food intake, energy expenditure, and whole-body energy balance in rodents and humans. Leptin has been a potential target for treating obesity. The plasma insulin response appears more closely associated with the plasma Leptin concentration. Neonatal Leptin levels are strongly associated with female gender, birth length, and formula feeding. Leptin concentrations were higher in women than in men. In women, serum Leptin was the most important predictor of myocardial infarction. In patients with angiographically confirmed coronary atherosclerosis, leptin is a novel predictor of future cardiovascular events independent of other risk factors, including lipid status and CRP. Leptin may also play an important role in the pathophysiology of osteoarthritis.
ab108879 Leptin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Leptin in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Leptin in less than 5 hours. A Murine monoclonal antibody specific for Leptin has been pre-coated onto a microplate. Leptin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for Leptin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Human Leptin Antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Leptin coated Microplate - 1 x 96 wells 1 unit Human Leptin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
- LeptinLeptin Murine Obesity Homolog Leptin Precursor Obesity FactorOBObese proteinObesity factorObesity homolog mouse Obesity murine homolog leptinOBS
Our Abpromise guarantee covers the use of ab108879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Leptin Human ELISA Kit (ab108879)
ab108879 has not yet been referenced specifically in any publications.