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Overview

  • Product nameLeptin Human ELISA KitSee all Leptin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 0.12 ng/ml
  • Range
    0.125 ng/ml - 32 ng/ml
  • Recovery

    = 99.5 %

  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Leptin, a 16 kDa protein secreted from white adipocytes, has been implicated in the regulation of food intake, energy expenditure, and whole-body energy balance in rodents and humans. Leptin has been a potential target for treating obesity. The plasma insulin response appears more closely associated with the plasma Leptin concentration. Neonatal Leptin levels are strongly associated with female gender, birth length, and formula feeding. Leptin concentrations were higher in women than in men. In women, serum Leptin was the most important predictor of myocardial infarction. In patients with angiographically confirmed coronary atherosclerosis, leptin is a novel predictor of future cardiovascular events independent of other risk factors, including lipid status and CRP. Leptin may also play an important role in the pathophysiology of osteoarthritis.

    ab108879 Leptin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Leptin in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Leptin in less than 5 hours. A Murine monoclonal antibody specific for Leptin has been pre-coated onto a microplate. Leptin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for Leptin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P41159 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        FLJ94114LEPLEP_HUMAN
        LeptinLeptin Murine Obesity Homolog Leptin Precursor Obesity FactorOBObese proteinObesity factorObesity homolog mouse Obesity murine homolog leptinOBS
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108879 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Leptin Human ELISA Kit images

      • Sandwich ELISA - Leptin Human ELISA Kit - 1 x 96 Well Plate (ab108879)
        Sandwich ELISA - Leptin Human ELISA Kit - 1 x 96 Well Plate (ab108879)
        Human Leptin Standard Curve

      Protocols

      References for Leptin Human ELISA Kit (ab108879)

      ab108879 has not yet been referenced specifically in any publications.

      Publishing research using ab108879 ? Please let us know so that we can cite the reference in this datasheet

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      What is the difference between products: ab 108879 and ab100581?

      These products are very similar, they use similar techniques to measure human Leptin in samples. In reviewing the information about each kit I have noted that ab100581 Sensitivity< 2 pg/ml, Range of 1.64 pg/ml - 400 pg/ml and Recovery of 94%. While ab1...

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      I would like to measure Leptin in either plasma or serum of pigs. I would like to know the chances of one of the abcam available leptin ELISA kits to work in pigs. Find below the pig leptin aminoacid sequence
      MRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTIVTR...

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      Thank you very much for your inquiry and your interest in our kits.

      Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

      For the other three kits, ab100581, ab100718 and ab100773,the laboratory...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"