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Overview

  • Product nameLeptin Mouse ELISA KitSee all Leptin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 4 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.47 83% - 103%
    Serum 93.29 82% - 102%
    Plasma 95.38 84% - 104%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Leptin is a secreted protein of 16 kDa. Human and Murine leptin show approximately 84 percent identity at the protein level. Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Studies indicate that Human obesity may be associated with leptin receptor deficiencies rather than constituting a problem with leptin itself.

    ab100718 Mouse Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Leptin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Mouse Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml
    Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody Leptin: biotinylated anti-mouse Leptin 2 vials
    HRP-Streptavidin Concentrate 120x concentrated HRP-conjugated streptavidin 1 x 200µl
    Leptin Microplate (12 strips x 8 wells) coated with anti-Mouse Leptin 1 unit
    Leptin Standard Mouse (recombinant) 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P41159 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        FLJ94114LEPLEP_HUMAN
        LeptinLeptin Murine Obesity Homolog Leptin Precursor Obesity FactorOBObese proteinObesity factorObesity homolog mouse Obesity murine homolog leptinOBS
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100718 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Leptin Mouse ELISA Kit images

      • ELISA - Leptin Mouse ELISA Kit (ab100718)
        ELISA - Leptin Mouse ELISA Kit (ab100718)

      Protocols

      References for Leptin Mouse ELISA Kit (ab100718)

      ab100718 has not yet been referenced specifically in any publications.

      Publishing research using ab100718 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100718 Submit a Question for ab100718

      Displaying 1 - 3 of 3 results for Abreviews and Q&A

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      Votes: Highest First

      I would like to measure Leptin in either plasma or serum of pigs. I would like to know the chances of one of the abcam available leptin ELISA kits to work in pigs. Find below the pig leptin aminoacid sequence
      MRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTIVTR...

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      Thank you very much for your inquiry and your interest in our kits.

      Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

      For the other three kits, ab100581, ab100718 and ab100773,the laboratory...

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      What type of graph is required for the results from this kit, ab100718?

      Thank you for your enquiry.

      I have now received further information regarding the reading of the results from this kit. The manuals advise using a log-log model as this type of plot will fit most data sets very well. However, you can use any gr...

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      Can this kit be used with blood samples? How much of my sample should I start with? Is there a suggested dilution/concentration?

      Thank you for your inquiry.  This kit ab100718 has been validated for serum, plasma, and cell culture supernatant samples. Are you using whole blood samples?  If so, this kit may work, but we would not recommend it as the chances of experiencing high ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"