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Recombinant fragment: LGGVLEASGY STEVV, corresponding to amino acids 131-145 of Human Leptin.
Our Abpromise guarantee covers the use of ab20101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||1/200. Can be paired for Sandwich ELISA with Rabbit polyclonal to Leptin (ab7208).
For sandwich ELISA, use this antibody as Capture with ab7208 as Detection.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/4000. Detects a band of approximately 16 kDa. Dilution optimised using Chromogenic detection.|
|IHC-Fr||Use at an assay dependent concentration.|
Standard curve for Leptin (Analyte: ab51273); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [BDI170] to Leptin (ab20101) at 1/200 dilution and Detector Antibody Rabbit polyclonal to Leptin (ab7208) at 0.5µg/ml. Concentration of Mouse monoclonal [BDI170] to Leptin (ab20101) may vary from lot to lot; please use this curve as guideline.
ab20101 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"