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Anti-Leptin antibody [BDI170] (ab20101)

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Overview

  • Product nameAnti-Leptin antibody [BDI170]See all Leptin primary antibodies ...
  • Description
    Mouse monoclonal [BDI170] to Leptin
  • SpecificityBinds to the carboxyl terminal region of the protein and to the intact leptin. Does not cross react with Epidermal Growth Factor (Human), Insulin (Human), Insulin-like Growth Factor 1 (Human), Insulin-like Growth Factor 2 (Rat), Parathyroid hormone (Human), Transforming Growth Factor-alpha (Human).
  • Tested applicationsSandwich ELISA, ICC/IF, WB, IHC-Fr more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: LGGVLEASGY STEVV, corresponding to amino acids 131-145 of Human Leptin.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHuman adipocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab20101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA: Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Leptin (ab7208). For sandwich ELISA, use this antibody as Capture with ab7208 as Detection.
ICC/IF ICC/IF: Use at an assay dependent dilution.
WB WB: 1/4000. Detects a band of approximately 16 kDa. Dilution optimised using Chromogenic detection.
IHC-Fr IHC-Fr: Use at an assay dependent dilution.

Target

  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P41159 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        FLJ94114 antibodyLEP antibodyLEP_HUMAN antibody
        LEPD antibodyLeptin (murine obesity homolog) antibodyLeptin (obesity homolog, mouse) antibodyLeptin antibodyLeptin Murine Obesity Homolog antibodyLeptin Precursor Obesity Factor antibodyOB antibodyObese Protein antibodyObese, mouse, homolog of antibodyObesity antibodyObesity factor antibodyObesity factor antibodyObesity homolog mouse antibodyObesity Murine Homolog Leptin antibodyOBS antibodyOTTHUMP00000212285 antibody
      see all

    Anti-Leptin antibody [BDI170] images

    Protocols

    References for Anti-Leptin antibody [BDI170] (ab20101)

    ab20101 has not yet been referenced specifically in any publications.

    Publishing research using ab20101 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab20101 Submit a Question for ab20101

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    Hello,

    Weneedtwo different types ofanti-leptin antibodies for human milk samples, that each ones recognize different epitopes; and a third antibody anti-Ig against the animal where the anti-leptin antibodies were produced. Wich of yours product...

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    Thank you for contacting us and your interest in our products.

    We have a number of different antibodies directed against the human Leptin protein. The ideal choices for your experiment would depend on what application you are hoping to use.
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    Greetings- I would like to kindly ask you to help me select the  appropriate Elisa tests for the following products:   immunoglobulin G and A autoantibodies against ghrelin, orexin, leptin, insulin, neuropeptide Y,  peptide YY, ACTH and  alfa MCH.   Thank...

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    Thank you for contacting us. Please choose antibodies from the following catalogue numbers: anti Ghrelin ab48285, ab117620, ab15861, ab57222, ab104307 These antibodies are tested in Indirect ELISA so are guaranteed for this application and appli...

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    We have another inquiry Is is possible that this product (ab20101) contains other type of inmunoglobulins IgM or only others IgGs? Thank you in advance for your help 

    Thank you for your inquiry. Yes, since ab20101 to Leptin is ascites, you are correct in assuming that this product may contain other immunoglobulins including IgM and other IgGs.  It may also contain other mouse proteins. I hope this information hel...

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    I am interested in make immunoassay sELISA as indicate in ab20101 sheet. And I have a question, referred to the following paragraph, where it says that used capture antibody ab20101 at 5 µg/mL but in the same sheet says that the concentration not determin...

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    Thank you for your inquiry and your patience with this reply. Since this is ascites and not a purified antibody, we recommend testing multiple dilutions starting at a low dilution and using the graph online as a guideline. The exact concentration of ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"