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Overview

  • Product nameLeptin proteinSee all Leptin proteins and peptides ...
  • Protein descriptionRecombinant full length Leptin protein (Human) MW 16kDa, 147 amino acids.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesRecombinant human Leptin was overexpressed as insoluble protein aggregate in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Sequence notesMVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab51273 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab51273 can be used as a WB positive control in conjunction with ab16227.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab3583.

    • Protein info

    • Alternative names
        FLJ94114LEPLEP_HUMAN
        LEPDLeptinLeptin (murine obesity homolog)Leptin (obesity homolog, mouse)Leptin Murine Obesity HomologLeptin Precursor Obesity FactorOBObese ProteinObese, mouse, homolog ofObesityObesity factorObesity homolog mouseObesity Murine Homolog LeptinOBSOTTHUMP00000212285
      see all
  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P41159 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Leptin protein images

    • Sandwich ELISA - Leptin protein (ab51273)
      Sandwich ELISA - Leptin protein (ab51273)
      Standard curve for Leptin (Analyte: ab51273); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [BDI170] to Leptin (ab20101) at 5µg/ml and Detector Antibody Rabbit polyclonal to Leptin (ab7208) at 0.5µg/ml. Concentration of Mouse monoclonal [BDI170] to Leptin (ab20101) may vary from lot to lot; please use this curve as guideline.
    • SDS-PAGE - Leptin protein (ab51273)
      SDS-PAGE - Leptin protein (ab51273)
      ab51273 on 14% SDS-PAGE
    • Western blot - Anti-Leptin antibody (ab16227)
      Western blot - Anti-Leptin antibody (ab16227)
      Anti-Leptin antibody (ab16227) at 1/500 dilution + Leptin protein (ab51273) at 1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 3 minutes

    References for Leptin protein (ab51273)

    ab51273 has not yet been referenced specifically in any publications.

    Publishing research using ab51273 ? Please let us know so that we can cite the reference in this datasheet

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