Leptin protein (ab51273)
Constituents: PBS, pH 7.4
Our Abpromise guarantee covers the use of ab51273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use at an assay dependent concentration. Ab51273 can be used as a WB positive control in conjunction with ab16227.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
- LEPDLeptinLeptin (murine obesity homolog)Leptin (obesity homolog, mouse)Leptin Murine Obesity HomologLeptin Precursor Obesity FactorOBObese ProteinObese, mouse, homolog ofObesityObesity factorObesity homolog mouseObesity Murine Homolog LeptinOBSOTTHUMP00000212285
Leptin protein images
Standard curve for Leptin (Analyte: ab51273); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [BDI170] to Leptin (ab20101) at 5µg/ml and Detector Antibody Rabbit polyclonal to Leptin (ab7208) at 0.5µg/ml. Concentration of Mouse monoclonal [BDI170] to Leptin (ab20101) may vary from lot to lot; please use this curve as guideline.
ab51273 on 14% SDS-PAGE
Anti-Leptin antibody (ab16227) at 1/500 dilution +
Leptin protein (ab51273) at 1 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 3 minutes
References for Leptin protein (ab51273)
ab51273 has not yet been referenced specifically in any publications.