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Products:Signal Transduction >> Growth Factors/Hormones >> Hormones
Overview
Product name
Anti-Leu Enkephalin antibody
See all Leu Enkephalin products (3) ...
Description
Rabbit polyclonal to Leu Enkephalin
Specificity
Reacts with a wide range of other species. Leucine enkephalin, partial reactivity with methionine enkephalin, no detected reactivity with Substance P, VIP and CGRP.
Tested applications
IHC-R, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide conjugated to KLH by a Glutaraldehyde linker.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Leu Enkephalin antibody(ab22619)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-R
IHC-Fr
- 1 Image
IHC-P
Application notes
IHC-Fr: 1/500 - 1/4000.
IHC-P: 1/500 - 1/4000.
IHC-R: 1/500 - 1/4000.
Using overnight incubation and ABC/PAP procedure. Dilutions up to 1/10,000 have been achieved under optimized conditions. 1/200 to 1/4000, using overnight incubation and indirect immunofluorescence procedure. All standard crosslinking agents may be used, but formaldehyde based solutions are recommended.
Frozen sections and Vibratome slices are recommended for tract tracing.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Enkephalin and enkephalin-like peptides have been detected in many animal species. In mammals these opioid pentapeptides are cleaved from a common precursor molecule which is expressed in a number of cell types, including neurons and endocrine cells associated with the sympathetic nervous system where they are often co-stored with catecholamines. They are known to be endogenous ligands for Sopioid receptors and are known to mediate analgesia at the spinal level.
Cellular localization
Secreted
Alternative names
- Met enkephalin antibody
- Met enkephalin Arg Gly Leu antibody
- Met enkephalin Arg Phe antibody
see all
Database links
- Entrez Gene: 5179 Human
- Entrez Gene: 29237 Rat
- Omim: 131330 Human
- SwissProt: P01210 Human
- SwissProt: P04094 Rat
- Unigene: 339831 Human
Anti-Leu Enkephalin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Leu Enkephalin antibody(ab22619)
ab22619 (4µg/ml) staining leu enkephalin in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of seminal vesicles.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-Leu Enkephalin antibody (ab22619)
ab22619 has not yet been referenced specifically in any publications.
Publishing research using ab22619? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"