Leu Enkephalin antibody (ab22619)

Product Name 

Leu Enkephalin antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Leu Enkephalin

Immunogen 

Synthetic peptide conjugated to KLH by a Glutaraldehyde linker.

Species Reactivity 

Reacts with a wide range of species.
Not yet tested in other species.

Specificity 

Leucine enkephalin, partial reactivity with methionine enkephalin, no detected reactivity with Substance P, VIP and CGRP.

Tested applications 
(see key)

IHC-Fr, IHC-P, IHC-R

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-Fr: 1/500 - 1/4000.
IHC-P: 1/500 - 1/4000.
IHC-R: 1/500 - 1/4000.

Using overnight incubation and ABC/PAP procedure. Dilutions up to 1/10,000 have been achieved under optimized conditions. 1/200 to 1/4000, using overnight incubation and indirect immunofluorescence procedure. All standard crosslinking agents may be used, but formaldehyde based solutions are recommended.
Frozen sections and Vibratome slices are recommended for tract tracing.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Signal Transduction >> Growth Factors/Hormones >> Hormones

Relevance 

Enkephalin and enkephalin-like peptides have been detected in many animal species. In mammals these opioid pentapeptides are cleaved from a common precursor molecule which is expressed in a number of cell types, including neurons and endocrine cells associated with the sympathetic nervous system where they are often co-stored with catecholamines. They are known to be endogenous ligands for Sopioid receptors and are known to mediate analgesia at the spinal level.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: Whole serum

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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