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Product name
Anti-Lgi1 antibody
See all Lgi1 products (3) ...
Description
Rabbit polyclonal to Lgi1
Tested applications
IHC-FoFr, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Hamster, Human
Predicted to work with
Chicken, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Lgi1.
(Peptide available as ab331 14.)
Positive control
Mouse Brain Whole Tissue Lysate, Mouse Brain (0 day) Whole Tissue Lysate and Rat Brain Whole Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: 1/100.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ICC/IF: Use a concentration of 10 µg/ml.
Target
Function
Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
Tissue specificity
Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
Involvement in disease
Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
Sequence similarities
Contains 7 EAR repeats.
Contains 3 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Post-translational
modifications
Glycosylated.
Cellular localization
Secreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum.
Target information above from: UniProt accessionO95970
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Epitempin 1 antibody
- EPITEMPIN antibody
- Epitempin-1 antibody
see all
Database links
- Entrez Gene: 423802 Chicken
- Entrez Gene: 9211 Human
- Entrez Gene: 56839 Mouse
- Entrez Gene: 252892 Rat
- Omim: 604619 Human
- SwissProt: Q5E9T6 Cow
- SwissProt: O95970 Human
- SwissProt: Q9JIA1 Mouse
see all
Anti-Lgi1 antibody images:
Western blot - Lgi1 antibody (ab30868)
Lane 1 : Marker
Lanes 2 - 4 : Anti-Lgi1 antibody (ab30868) at 1 µg/ml
Lane 1 : As above
Lane 2 : Mouse Brain Whole Tissue Lysate at 20 µg
Lane 3 :
Lane 4 : Rat Brain Whole Tissue Lysate at 20 µg
Secondary
Lanes 2 - 4 : IR Dye 680 Conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 64 kDa
Observed band size : 64 kDa
Immunocytochemistry/ Immunofluorescence - Lgi1 antibody (ab30868)
ab30868 at 10ug/ml staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized and blocked before incubation with the antibody for 16 hours. An Alexa Fluor ® 488 conjugated goat polyclonal antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (PFA perfusion fixed frozen sections) - Lgi1 antibody (ab30868)
IHC-FoFr image of Lgi1 (ab30868) staining in rat spinal cord sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique. Image B is a higher power magnification of the Image A, at the level of the interface between the white and grey matter in the lamina V. Numbers in image A are Rexed's laminae numbers.
Sophie Pezet, ESPCI, France
References for Anti-Lgi1 antibody (ab30868)
This product has been referenced in:
- Zhou YD et al. Epilepsy gene LGI1 regulates postnatal developmental remodeling of retinogeniculate synapses. J Neurosci 32:903-10 (2012). WB; Mouse.Read more (PubMed: 22262888) »
- Fukata Y et al. Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy. Proc Natl Acad Sci U S A 107:3799-804 (2010).Read more (PubMed: 20133599) »
See all 6 publications for this product
Publishing research using ab30868? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"