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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab75483? Please let us know so that we can cite the reference in this datasheet
ab75483 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lin28 antibody [55CT58.12.1] (ab75483)](/ps/datasheet/Images/75/ab75483/ab75483ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lin28 antibody [55CT58.12.1] (ab75483)
Formalin-fixed and paraffin-embedded human testis tissue reacted with ab75483 at a dilution of 1/50. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.
![Western blot - Lin28 antibody [55CT58.12.1] (ab75483)](/ps/datasheet/images/75/ab75483/Lin28-Primary-antibodies-ab75483-2.jpg)
Western blot - Lin28 antibody [55CT58.12.1] (ab75483)
All lanes : Anti-Lin28 antibody [55CT58.12.1] (ab75483) at 1 µg/ml
Lane 1 :
Lane 2 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
The predicted molecular weight of Lin28 is 23 kDa (SwissProt), however we expect to observe a banding pattern at 28 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
![Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody [55CT58.12.1] (ab75483)](/ps/datasheet/images/75/ab75483/Lin28-Primary-antibodies-ab75483-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody [55CT58.12.1] (ab75483)
ICC/IF image of ab75483 stained MES cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75483, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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