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Anti-Lin28 antibody [55CT58.12.1] (ab75483)

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Overview

Product name

Anti-Lin28 antibody [55CT58.12.1]
See all Lin28 products (11) ...

Description

Mouse monoclonal [55CT58.12.1] to Lin28

Tested applications

ICC/IF, WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Lin28 recombinant protein is used to produce this monoclonal antibody.

Positive control

Testis tissue

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Clonality

Monoclonal

Clone number

55CT58.12.1

Isotype

IgG1

Light chain type

kappa

Research areas

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Epigenetics and Nuclear Signaling >> RNAi >> miRNA processing
Stem Cells >> Embryonic Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lin28 antibody [55CT58.12.1] (ab75483)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lin28 antibody [55CT58.12.1] (ab75483) image (enlarge)

  • Western blot - Lin28 antibody [55CT58.12.1] (ab75483)Western blot - Lin28 antibody [55CT58.12.1] (ab75483) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody [55CT58.12.1] (ab75483)Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody [55CT58.12.1] (ab75483) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

Tissue specificity

Expressed in embryonic stem cells (ES cells), placenta and testis.

Sequence similarities

Belongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain.

Developmental stage

Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.

Domain

The CSD domain is required for function in muscle differentiation.

Cellular localization

Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.

Target information above from: UniProt accessionQ9H9Z2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AL024421 antibody
  • CSDD1 antibody
  • FLJ12457 antibody
  • Lin 28 antibody
  • Lin 28 homolog (C. elegans) antibody
  • Lin 28 homolog A antibody
  • Lin 28 homolog antibody
  • Lin-28 antibody
  • Lin-28 homolog (C. elegans) antibody
  • Lin-28 homolog A antibody
  • Lin-28A antibody
  • Lin28 antibody
  • Lin28a antibody
  • LN28A_HUMAN antibody
  • Protein lin-28 homolog A antibody
  • RNA binding protein lin 28 antibody
  • RNA binding protein lin-28 antibody
  • Tex17 antibody
  • ZCCHC1 antibody
  • Zinc finger CCHC domain containing 1 antibody
  • Zinc finger CCHC domain containing protein 1 antibody
  • Zinc finger CCHC domain-containing protein 1 antibody
see all

Database links

Anti-Lin28 antibody [55CT58.12.1] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lin28 antibody [55CT58.12.1] (ab75483)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lin28 antibody [55CT58.12.1] (ab75483)

Formalin-fixed and paraffin-embedded human testis tissue reacted with ab75483 at a dilution of 1/50. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.

  Western blot - Lin28 antibody [55CT58.12.1] (ab75483)

Western blot - Lin28 antibody [55CT58.12.1] (ab75483)

All lanes : Anti-Lin28 antibody [55CT58.12.1] (ab75483) at 1 µg/ml

Lane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 23 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 12 minutes

The predicted molecular weight of Lin28 is 23 kDa (SwissProt), however we expect to observe a banding pattern at 28 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody [55CT58.12.1] (ab75483)

Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody [55CT58.12.1] (ab75483)

ICC/IF image of ab75483 stained MES cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75483, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Lin28 antibody [55CT58.12.1] (ab75483)

ab75483 has not yet been referenced specifically in any publications.

Publishing research using ab75483? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"