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Western blot - Lin28 antibody (ab46020)
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Immunocytochemistry/ Immunofluorescence - Lin28 antibody (ab46020)
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Product Name
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Lin28 antibody
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See all Lin28 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Lin28
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Immunogen
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Recombinant full length protein (Human) with hexahistidine tag
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Reacts with
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Hu, Ms
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Tested applications
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/7000. (PMID 20101213)
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic, nuclear and nucleolar.
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Chromatin and Nuclear Signaling >> RNAi >> miRNA processing
Stem Cells >> Embryonic Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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Lin28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. Lin28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Lin28 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Lin28 antibody images, references, products related to ab46020 and other tools.
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Lin28 antibody images:
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 Western blot - Lin28 antibody (ab46020)
All lanes : Lin28 antibody (ab46020) at 1/1000 dilution
Lane 1 : PC-3 cell extract in lysis buffer containing NP-40 at 45 µg
Lane 2 : PC-3 cell extract (Trizol bottom phase) at 30 µg
Lane 3 : Tera-2 cell extract (Trizol bottom phase) at 30 µg
Lane 4 : Tera-2 cell extract in lysis buffer containing NP-40 at 45 µg
Secondary
Goat anti-rabbit HRP conjugated at 1/4000 dilution
Predicted band size : 23 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
It is unclear why lin28 runs at a larger size than its predicted moleular weight but recombinant lin28 protein is also known to run at around 30 kDa.
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Immunocytochemistry/ Immunofluorescence - Lin28 antibody (ab46020)
Human Embryonic stem cells were fixed in PFA and then incubated with the primary antibody ab46020 (1:1000) diluted in 2% BSA for 16 hours at 4C. Cells where then washed and incubated with a Cy3 conjugated goat anti-rabbit polyclonal antibody (1:400). Cells where then washed and visualised.
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References for Lin28 antibody (ab46020)
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This product has been used in: (two most recent references)
Peng S et al. Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer. Oncogene : (2010). WB, ICC/IF, IHC-P; Human. PubMed: 20101213
Melton C et al. Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature 463:621-6 (2010). WB; Mouse. PubMed: 20054295
See all 8 publication references for this product.
If you publish research using ab46020 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Lin28
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Lin28 antibody - more information
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Customer reviews (feedback) regarding Lin28 antibody |
Customer FAQs regarding Lin28 antibody |
Protocols for Lin28 antibody |
Price and availability of products related to Lin28 antibody |
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Lin28 antibody - related products:
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Lin28 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Lin28 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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