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Western blot - Lin28 antibody (ab46020)
Western blot - Lin28 antibody (ab46020)
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Immunocytochemistry/ Immunofluorescence - Lin28 antibody (ab46020)
Immunocytochemistry/ Immunofluorescence - Lin28 antibody (ab46020)
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Product Name 

Lin28 antibody

 

See all Lin28 antibodies (7)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Lin28

Immunogen 

Recombinant full length protein (Human) with hexahistidine tag

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


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• ICC/IF

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• WB

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


ICC/IF
(read Abreview)


WB
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/7000. (PMID 20101213)
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic, nuclear and nucleolar.

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Chromatin and Nuclear Signaling >> RNAi >> miRNA processing
Stem Cells >> Embryonic Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis

Relevance 

Lin28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. Lin28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

 

Alternative names Lin28 antibody (ab46020)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

79727    (Human)
83557    (Mouse)

Omim

    

611043    (Human)

SwissProt

    

Q9H9Z2    (Human)
Q8K3Y3    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Lin28 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Lin28 antibody images, references, products related to ab46020 and other tools.

 

Lin28 antibody images:

Product image  Western blot - Lin28 antibody (ab46020)

Western blot - Lin28 antibody (ab46020)

All lanes : Lin28 antibody (ab46020) at 1/1000 dilution

Lane 1 : PC-3 cell extract in lysis buffer containing NP-40 at 45 µg
Lane 2 : PC-3 cell extract (Trizol bottom phase) at 30 µg
Lane 3 : Tera-2 cell extract (Trizol bottom phase) at 30 µg
Lane 4 : Tera-2 cell extract in lysis buffer containing NP-40 at 45 µg

Secondary
Goat anti-rabbit HRP conjugated at 1/4000 dilution

Predicted band size : 23 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

It is unclear why lin28 runs at a larger size than its predicted moleular weight but recombinant lin28 protein is also known to run at around 30 kDa.

 

Product image  Immunocytochemistry/ Immunofluorescence - Lin28 antibody (ab46020)

Immunocytochemistry/ Immunofluorescence - Lin28 antibody (ab46020)

Human Embryonic stem cells were fixed in PFA and then incubated with the primary antibody ab46020 (1:1000) diluted in 2% BSA for 16 hours at 4C. Cells where then washed and incubated with a Cy3 conjugated goat anti-rabbit polyclonal antibody (1:400). Cells where then washed and visualised.

 

References for Lin28 antibody (ab46020)

Specific publication references   This product has been used in: (two most recent references)

Peng S et al. Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer. Oncogene : (2010). WB, ICC/IF, IHC-P; Human. PubMed: 20101213

Melton C et al. Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature 463:621-6 (2010). WB; Mouse. PubMed: 20054295


See all 8 publication references for this product.


If you publish research using ab46020 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Lin28

 

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