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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Lin28 antibody
See all Lin28 products (11) ...
Description
Rabbit polyclonal to Lin28
Tested applications
WB, IHC-P, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein (Human) with hexahistidine tag
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab46020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
Application notes
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/7000. (PMID 20101213)
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Tissue specificity
Expressed in embryonic stem cells (ES cells), placenta and testis.
Sequence similarities
Belongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain.
Developmental stage
Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
Domain
The CSD domain is required for function in muscle differentiation.
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
Target information above from: UniProt accessionQ9H9Z2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AL024421 antibody
- CSDD1 antibody
- FLJ12457 antibody
see all
Database links
- Entrez Gene: 79727 Human
- Entrez Gene: 83557 Mouse
- Omim: 611043 Human
- SwissProt: Q9H9Z2 Human
- SwissProt: Q8K3Y3 Mouse
- Unigene: 86154 Human
- Unigene: 302567 Mouse
Anti-Lin28 antibody images:
Western blot - Lin28 antibody (ab46020)
All lanes : Anti-Lin28 antibody (ab46020) at 1/1000 dilution
Lane 1 : PC-3 cell extract in lysis buffer containing NP-40 at 45 µg
Lane 2 : PC-3 cell extract (Trizol bottom phase) at 30 µg
Lane 3 : Tera-2 cell extract (Trizol bottom phase) at 30 µg
Lane 4 : Tera-2 cell extract in lysis buffer containing NP-40 at 45 µg
Secondary
Goat anti-rabbit HRP conjugated at 1/4000 dilution
Predicted band size : 23 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
It is unclear why lin28 runs at a larger size than its predicted moleular weight but recombinant lin28 protein is also known to run at around 30 kDa.
Immunocytochemistry/ Immunofluorescence - Lin28 antibody (ab46020)
Human Embryonic stem cells were fixed in PFA and then incubated with the primary antibody ab46020 (1:1000) diluted in 2% BSA for 16 hours at 4C. Cells where then washed and incubated with a Cy3 conjugated goat anti-rabbit polyclonal antibody (1:400). Cells where then washed and visualised.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lin28 antibody (ab46020)
ab46020 staining Lin28 in paraffin-embedded human tissue sections (ESC-derived embryoid bodies) by IHC-P. Tissue was fixed with paraformaldehyde and blocked with 8% Serum treatment in automated Bond Max system for 15 minutes. Samples were incubated with primary antibody (1/2000) for 15 minutes. An HRP-conjugated goat anti rabbit polyclonal (1/200) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-Lin28 antibody (ab46020)
This product has been referenced in:
- Nadiminty Net al. MicroRNA let-7c Suppresses Androgen Receptor Expression and Activity via Regulation of Myc Expression in Prostate Cancer Cells. J Biol Chem 287:1527-37 (2012). WB; Human.Read more (PubMed: 22128178) »
- Jin Jet al. Evidence that Lin28 stimulates translation by recruiting RNA helicase A to polysomes. Nucleic Acids Res 39:3724-34 (2011). IP; Human.Read more (PubMed: 21247876) »
See all 13 publications for this product
Publishing research using ab46020? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"