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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab63740 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - Lin28 antibody (ab63740)
ICC/IF image of ab63740 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63740, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Western blot - Lin28 antibody (ab63740)
All lanes : Anti-Lin28 antibody (ab63740) at 1 µg/ml
Lane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
Lane 3 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 4 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
The 30 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to Lin28.
Sandwich ELISA - Lin28 antibody (ab63740)
Standard Curve for Lin28 (Analyte:
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