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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Lin28 antibody
See all Lin28 products (11) ...
Description
Rabbit polyclonal to Lin28
Tested applications
Sandwich ELISA, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Horse, Pig, Chimpanzee
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Lin28.
(Peptide available as ab64578.)
Positive control
This antibody gave a positive signal in the following Whole Cell Lysates: MEL-1 F9 ES-E14TG2a Mouse EG
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 0.1 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [6D1F9] to Lin28 (ab76369). (For sandwich ELISA, use this antibody as Detection at 0.1 µg/ml with Mouse monoclonal [6D1F9] to Lin28 (ab76369) as Capture.)
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
Target
Function
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Tissue specificity
Expressed in embryonic stem cells (ES cells), placenta and testis.
Sequence similarities
Belongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain.
Developmental stage
Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
Domain
The CSD domain is required for function in muscle differentiation.
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
Target information above from: UniProt accessionQ9H9Z2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AL024421 antibody
- CSDD1 antibody
- FLJ12457 antibody
see all
Database links
- Entrez Gene: 79727 Human
- Entrez Gene: 83557 Mouse
- Entrez Gene: 500562 Rat
- Omim: 611043 Human
- SwissProt: Q9H9Z2 Human
- SwissProt: Q8K3Y3 Mouse
- Unigene: 86154 Human
- Unigene: 302567 Mouse
Anti-Lin28 antibody images:
Immunocytochemistry/ Immunofluorescence - Lin28 antibody (ab63740)
ICC/IF image of ab63740 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63740, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Western blot - Lin28 antibody (ab63740)
All lanes : Anti-Lin28 antibody (ab63740) at 1 µg/ml
Lane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
Lane 3 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 4 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
The 30 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to Lin28.
Sandwich ELISA - Lin28 antibody (ab63740)
Standard Curve for Lin28 (Analyte: Lin28 protein (His tag) (ab89225)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [6D1F9] to Lin28 (ab76369) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Lin28 (ab63740) at 0.1 ug/ml.
Protocols
References for Anti-Lin28 antibody (ab63740)
ab63740 has not yet been referenced specifically in any publications.
Publishing research using ab63740? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"