Lin28B antibody (ab71415)

Product Name 

Lin28B antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Lin28B

Immunogen 

Synthetic peptide between amino acids of 105-135 of human Lin28B, conjugated to KLH. The peptide has 80% homology for LIN28A (Q9H9Z2).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/10 - 1/50.
WB: 1/100 - 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

K562 cell line lysates.

Cellular localization 

Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively.

Research areas 

Cancer >> Tumor biomarkers >> Other
Chromatin and Nuclear Signaling >> RNAi >> miRNA processing

Relevance 

Lin28B acts as a suppressor of microRNA (miRNA) biogenesis. Lin28B binds to the miRNA precursor pre-let-7 and recruits the uridylyltransferase ZCCHC11/TUT4, resulting in the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs are not processed by Dicer and are subsequently degraded. Lin28B recognises the 5'-GGAG-3' motif present in the terminal loop of pre-let-7. It also recognizes, and binds to, other pre-miRNAs that contain this motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Lin28B mediates Myc-mediated let-7 repression. Overexpression of Lin28B has been observed in many cancers, and is linked to repression of the let-7 family of miRNAs and derepression of let-7 targets.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Ammonium Sulphate Precipitation

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Lin28B antibody images, references, products related to ab71415 and other tools.

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References for Lin28B antibody (ab71415)

  This product has been used in:

Iliopoulos D et al. An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation. Cell 139:693-706 (2009). WB, ChIP; Human. PubMed: 19878981

If you publish research using ab71415 please let us know so that we can cite the reference on this datasheet.

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