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Products:Epigenetics and Nuclear Signaling >> RNAi >> miRNA processing
Overview
Product name
Anti-Lin28B antibody
See all Lin28B products (3) ...
Description
Rabbit polyclonal to Lin28B
Tested applications
IHC-P, Flow Cyt, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide between amino acids of 105-135 of human Lin28B, conjugated to KLH. The peptide has 80% homology for LIN28A (Q9H9Z2).
Positive control
K562 cell line lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Epigenetics and Nuclear Signaling >> RNAi >> miRNA processing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10 - 1/50.
Flow Cyt
Flow Cyt: 1/10 - 1/50.Flow Cyt: 1/10 - 1/50.
WB: 1/50 - 1/100.Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Tissue specificity
High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level).
Sequence similarities
Belongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain.
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively.
Target information above from: UniProt accessionQ6ZN17
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CSDD 2 antibody
- CSDD2 antibody
- FLJ16517 antibody
see all
Database links
- Entrez Gene: 389421 Human
- Omim: 611044 Human
- SwissProt: Q6ZN17 Human
- Unigene: 23616 Human
Anti-Lin28B antibody images:
Western blot - Lin28B antibody (ab71415)
Anti-Lin28B antibody (ab71415) at 1/60 dilution + K562 cell line lysates at 35 µg
Predicted band size : 27 kDa
Observed band size : 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Lin28B antibody(ab71415)
ab71415 at 1/50 dilution staining Lin28B in human testis tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-Lin28B antibody (ab71415)
This product has been referenced in:
- Li Xet al. MiR-181 mediates cell differentiation by interrupting the Lin28 and let-7 feedback circuit. Cell Death Differ : (2011). WB; Human.Read more (PubMed: 21979467) »
- Iliopoulos Det al. An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation. Cell 139:693-706 (2009). WB; Human.Read more (PubMed: 19878981) »
See all 2 publications for this product
Publishing research using ab71415? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"