Lipase Detection Kit III (Fluorometric) (ab118969)

Overview

  • Product nameLipase Detection Kit III (Fluorometric)See all Lipase kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.1 mU/well
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: all Mammals
  • Product overview

    In Abcam's Lipase Detection Kit III (Fluorometric), Lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Em/Ex=529/600 nm. This kit can be used to detect Lipase as low as 0.1 mU/well.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The kit provides a rapid, simple, more sensitive, and reliable test suitable for high throughput assay of Lipase activity.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceLipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.

Applications

Our Abpromise guarantee covers the use of ab118969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent concentration.

Lipase Detection Kit III (Fluorometric) images

  • Example of standard curve obtained using ab118969.
  • Positive Control and Background assayed using ab118969.

Protocols

References for Lipase Detection Kit III (Fluorometric) (ab118969)

ab118969 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

The substates for ab102525 and ab118969 are triglyceride derivatives.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"