Overview

  • Product nameLipid Peroxidation (MDA) Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Plasma, Cell culture extracts, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.1 nmol/well
  • Product overview

    Abcam's Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceLipid peroxidation refers to the oxidative degradation of lipids and is a well-defined mechanism of cellular damage. The formation of lipid peroxidation products leads to spread of free radical reactions leading to cell damage.

Applications

Our Abpromise guarantee covers the use of ab118970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Lipid Peroxidation (MDA) Assay Kit images

  • MDA Standard Curve

Protocols

References for Lipid Peroxidation (MDA) Assay Kit (ab118970)

This product has been referenced in:
  • Kalghatgi S  et al. Bactericidal antibiotics induce mitochondrial dysfunction and oxidative damage in Mammalian cells. Sci Transl Med 5:192ra85 (2013). Read more (PubMed: 23825301) »
  • Yeh JH  et al. Curcumin attenuates renal ischemia and reperfusion injury-induced restrictive respiratory insufficiency. Transplant Proc 45:3542-5 (2013). Read more (PubMed: 24314954) »

See all 6 Publications for this product

Product Wall



I double checked and unfortunately, have confirm that we do not have a kit for measuring NADPH oxidase in our catalog at the moment. We do offer ab65349 which is a NADP/NADPH Assay Kit:

http://www.abcam.com/index.html?datasheet=6534...

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Centrifuge the samples and take only the clear supernatant for the next step.

Thank you for your enquiry.

I can confirm that you will need to use the n-butanol for the standards as well, since it helps in isolating the MDA-TBA adduct.

I hope this information has been useful for you. Please do not hesitate t...

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Thank you for contacting us about this issue. The lab says the TBA generally goes into solution after mixing the TBA in acetic acid with the water. Did you see any apparent dissolving after mixing the two by vortexing? Did you try heating it in a fume ...

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Regarding ab133085, we do not have data for the collection, freezing and storage of plant tissue samples for 6 months at -80ºC. From your customer’s protocol, they did a good job of collecting, freezing and storing their tissues, but th...

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Thank you for your inquiry.

I heard from the lab that you can start with a greater number of cells and homogenize them in the same 300 ul MDA lysis buffer. They didn't think deproteinization would help. But you may want to use n-butanol to ex...

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Yes, that is correct: 0, 2, 4, 6, 8, 10 nmol per well, since you have used half the suggested standard volume. Reporting nmol/mg should be fine, but converting to ng/mg is easy enough to do. I am not sure why the other kit we discussed uses uM units bu...

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Here is my conversion from nanomoles to micromolar concentrations and micrograms of MDA for the units that are used for the X-axis of the data on the datasheet. Please let me know if this does not answer you question or if you have concerns about the v...

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Thank you for contacting us.


The laboratory does not recommend that the protocol be stopped for later continuation at any step for this assay. If you cannot perform the whole protocol on the same day, I would recommend storing the sampl...

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Thank you for your enquiry which has been forwarded to the scientific support team.

I am sorry to confirm this kit has not been tested on serum samples yet. Since it has not been used for analysis of serum, I am sorry we unfortunately do not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"