Lipid Peroxidation (MDA) Assay Kit (ab118970)
- Product nameLipid Peroxidation (MDA) Assay Kit
- Tests100 x 1 assay
- Sample typePlasma, Cell culture extracts, Tissue Extracts
- Assay typeQuantitative
- Sensitivity> 0.1 nmol/well
- Product overview
Abcam's Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
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Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests BHT (100X) Purple 1 x 1ml MDA Lysis Buffer WM 1 x 25ml MDA Standard (4.17M) Yellow 1 x 100µl Phosphotungstic Acid Solution NM 1 x 12.5ml TBA NM 4 vials
- RelevanceLipid peroxidation refers to the oxidative degradation of lipids and is a well-defined mechanism of cellular damage. The formation of lipid peroxidation products leads to spread of free radical reactions leading to cell damage.
Our Abpromise guarantee covers the use of ab118970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Lipid Peroxidation (MDA) Assay Kit (ab118970)
This product has been referenced in:
- Kalghatgi S et al. Bactericidal antibiotics induce mitochondrial dysfunction and oxidative damage in Mammalian cells. Sci Transl Med 5:192ra85 (2013). Read more (PubMed: 23825301) »
- An CH et al. TLR4 deficiency promotes autophagy during cigarette smoke-induced pulmonary emphysema. Am J Physiol Lung Cell Mol Physiol 303:L748-57 (2012). Read more (PubMed: 22983353) »