Anti-Lipin 1 antibody (ab181389)
Key features and details
- Rabbit polyclonal to Lipin 1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Lipin 1 antibody
See all Lipin 1 primary antibodies -
Description
Rabbit polyclonal to Lipin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Lipin 1 (C terminal). The exact sequence is proprietary. 18 amino acids.
Database link: Q14693 -
Positive control
- K562 cell lysate. Human small intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 99 kDa).
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ICC/IF |
Use a concentration of 20 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 99 kDa). |
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol. -
Tissue specificity
Abundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract. -
Involvement in disease
Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years. -
Sequence similarities
Belongs to the lipin family. -
Domain
Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity. -
Post-translational
modificationsPhosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 23175 Human
- Entrez Gene: 14245 Mouse
- Entrez Gene: 313977 Rat
- Omim: 605518 Human
- SwissProt: Q14693 Human
- SwissProt: Q91ZP3 Mouse
- Unigene: 467740 Human
- Unigene: 153625 Mouse
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Alternative names
- EC=3.1.3.4 antibody
- KIAA0188 antibody
- Lipin-1 antibody
see all
Images
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Anti-Lipin 1 antibody (ab181389) at 1 µg/ml + K562 whole cell lysate at 15 µg
Predicted band size: 99 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of Human small intestine tissue, staining Lipin 1 with ab181389 at 5 μg/ml.
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Immunfluorescence analysis of Human small intestine tissue, staining Lipin 1 with ab181389 at 20 μg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab181389 has been referenced in 7 publications.
- Ingram LM et al. Identification of lipidomic profiles associated with drug-resistant prostate cancer cells. Lipids Health Dis 20:15 (2021). PubMed: 33596934
- Baneux C et al. Phosphatidic acid metabolism regulates neuroendocrine secretion but is not under the direct control of lipins. IUBMB Life 72:533-543 (2020). PubMed: 31967386
- Wang H et al. Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes. J Cell Mol Med 24:1399-1412 (2020). PubMed: 31809000
- Dinarvand N et al. Expression and clinicopathological significance of lipin-1 in human breast cancer and its association with p53 tumor suppressor gene. J Cell Physiol 235:5835-5846 (2020). PubMed: 31970786
- Zhao Y et al. Gain-of-Function Mutations of SLC16A11 Contribute to the Pathogenesis of Type 2 Diabetes. Cell Rep 26:884-892.e4 (2019). PubMed: 30673611
- Peng Q et al. Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes. Aging (Albany NY) 11:11520-11540 (2019). PubMed: 31831718
- Cheng XY et al. miR-203 Inhibits Alcohol-Induced Hepatic Steatosis by Targeting Lipin1. Front Pharmacol 9:275 (2018). PubMed: 29670525