Anti-Lipoamide Dehydrogenase antibody [2D4] (ab124414)

Overview

• Product nameAnti-Lipoamide Dehydrogenase antibody [2D4]See all Lipoamide Dehydrogenase primary antibodies ...
• Description
  Mouse monoclonal [2D4] to Lipoamide Dehydrogenase
• Tested applicationsWB, IHC-P, Sandwich ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein, corresponding to amino acids 1-510 of Human Lipoamide Dehydrogenase with Propietary tag (AAH18696).

• Positive controlHuman Heart tissue, Recombinant Lipoamide Dehydrogenase protein

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.20
  Constituent: 99% PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number2D4
• IsotypeIgG3
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Amino Acids

Applications

Target

• FunctionLipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
• Involvement in diseaseNote=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
  Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
• Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
• Post-translational
  modificationsTyrosine phosphorylated.
• Cellular localizationMitochondrion matrix.

Target information above from: UniProt accession P09622 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1738 Human
  • Omim: 238331 Human
  • SwissProt: P09622 Human
  • Unigene: 131711 Human

• Alternative names
  Diaphorase antibodyDihydrolipoamide dehydrogenase antibodyDihydrolipoyl dehydrogenase antibody

  Dihydrolipoyl dehydrogenase mitochondrial antibodydld antibodyDLDH antibodyDLDH_HUMAN antibodyE3 antibodyE3 component of pyruvate dehydrogenase antibodyE3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibodyGCSL antibodyGlycine cleavage system L protein antibodyGlycine cleavage system protein L antibodyLAD antibodylipoamide dehydrogenase antibodyLipoamide reductase antibodyLipoyl dehydrogenase antibodymitochondrial antibodyOTTHUMP00000206744 antibodyOTTHUMP00000206746 antibodyOTTHUMP00000206748 antibodyOTTHUMP00000206749 antibodyPHE 3 antibodyPHE3 antibody

  see all

Anti-Lipoamide Dehydrogenase antibody [2D4] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoamide Dehydrogenase antibody [2D4] (ab124414)
  Staining of Lipoamide Dehydrogenase in Paraffin-embedded Human heart tissue by Immunohistochemistry using ab124414 at a concentration of 5 µg/ml.
• 

  Sandwich ELISA - Anti-Lipoamide Dehydrogenase antibody [2D4] (ab124414)
  Detection limit for recombinant Proprietary tagged Lipoamide Dehydrogenase is approximately 1ng/ml, using ab124414 as a capture antibody.