Anti-Lipoamide Dehydrogenase antibody [2D4] (ab124414)

Overview

  • Product nameAnti-Lipoamide Dehydrogenase antibody [2D4]See all Lipoamide Dehydrogenase primary antibodies ...
  • Description
    Mouse monoclonal [2D4] to Lipoamide Dehydrogenase
  • Tested applicationsWB, IHC-P, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-510 of Human Lipoamide Dehydrogenase with Propietary tag (AAH18696).

  • Positive control
    • Human Heart tissue, Recombinant Lipoamide Dehydrogenase protein

Properties

Applications

Our Abpromise guarantee covers the use of ab124414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa. Note: recombinant protein was used to test this antibody for WB
IHC-P Use a concentration of 5 µg/ml.
Sandwich ELISA Use at an assay dependent dilution.

Target

  • FunctionLipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
  • Involvement in diseaseNote=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
    Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
  • Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • Post-translational
    modifications
    Tyrosine phosphorylated.
  • Cellular localizationMitochondrion matrix.
  • Target information above from: UniProt accession P09622 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Diaphorase antibody
    • Dihydrolipoamide dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase mitochondrial antibody
    • dld antibody
    • DLDD antibody
    • DLDH antibody
    • DLDH_HUMAN antibody
    • E3 antibody
    • E3 component of pyruvate dehydrogenase antibody
    • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody
    • GCSL antibody
    • Glycine cleavage system L protein antibody
    • Glycine cleavage system protein L antibody
    • LAD antibody
    • lipoamide dehydrogenase antibody
    • Lipoamide reductase antibody
    • Lipoyl dehydrogenase antibody
    • mitochondrial antibody
    • OTTHUMP00000206744 antibody
    • OTTHUMP00000206746 antibody
    • OTTHUMP00000206748 antibody
    • OTTHUMP00000206749 antibody
    • PHE 3 antibody
    • PHE3 antibody
    see all

Anti-Lipoamide Dehydrogenase antibody [2D4] images

  • Staining of Lipoamide Dehydrogenase in Paraffin-embedded Human heart tissue by Immunohistochemistry using ab124414 at a concentration of 5 µg/ml.
  • Detection limit for recombinant Proprietary tagged Lipoamide Dehydrogenase is approximately 1ng/ml, using ab124414 as a capture antibody.

References for Anti-Lipoamide Dehydrogenase antibody [2D4] (ab124414)

ab124414 has not yet been referenced specifically in any publications.

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