Lipocalin-2 / NGAL Human ELISA Kit (ab113326)
- Product nameLipocalin-2 / NGAL Human ELISA KitSee all Lipocalin-2 / NGAL kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 4 pg/ml
- Range4.1 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 75.47 69% - 88% Serum 103.9 94% - 112% Plasma 109.3 96% - 130%
- Species reactivityReacts with: Human
- Product overview
ab113326 Human Lipocalin-2 / NGAL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Lipocalin-2 / NGAL in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human Lipocalin-2 / NGAL coated on a 96-well plate. Standards and samples are pipetted into the wells and Lipocalin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Lipocalin-2 / NGAL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin-2 / NGAL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 600X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent Item E 1 x 15ml Detection Antibody Lipocalin-2 / NGAL Item F 2 vials Lipocalin-2 / NGAL Microplate (12 strips x 8 wells) coated with anti-human Lipocalin-2 / NGAL Item A 1 unit Lipocalin-2 / NGAL Standard Human (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- 24p325 kDa alpha 2 microglobulin related subunit of MMP925 kDa alpha-2-microglobulin-related subunit of MMP-9
- Alpha 2 microglobulin related proteinHGNC:6526HNLHNLLcn 2Lcn2Lipocalin 2Lipocalin-2Migration stimulating factor inhibitorMSFINeutrophil gelatinase associated lipocalinNeutrophil gelatinase associated lipocalin precursorNeutrophil gelatinase-associated lipocalinNGALNGAL_HUMANOncogene 24p3p25SiderocalinSV40 induced 24P3 protein
Our Abpromise guarantee covers the use of ab113326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Lipocalin-2 / NGAL Human ELISA Kit (ab113326)
ab113326 has not yet been referenced specifically in any publications.