You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Lipocalin-2 / NGAL Human ELISA Kit (ab113326)

Submit an Abreview Q&A (6)

Overview

  • Product nameLipocalin-2 / NGAL Human ELISA KitSee all Lipocalin-2 / NGAL kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 4 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.47 69% - 88%
    Serum 103.9 94% - 112%
    Plasma 109.3 96% - 130%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab113326 Human Lipocalin-2 / NGAL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Lipocalin-2 / NGAL in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human Lipocalin-2 / NGAL coated on a 96-well plate. Standards and samples are pipetted into the wells and Lipocalin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Lipocalin-2 / NGAL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin-2 / NGAL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

    • Target information above from: UniProt accession P80188 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        24p325 kDa alpha 2 microglobulin related subunit of MMP925 kDa alpha-2-microglobulin-related subunit of MMP-9
        Alpha 2 microglobulin related proteinHGNC:6526HNLHNLLcn 2Lcn2Lipocalin 2Lipocalin-2Migration stimulating factor inhibitorMSFINeutrophil gelatinase associated lipocalinNeutrophil gelatinase associated lipocalin precursorNeutrophil gelatinase-associated lipocalinNGALNGAL_HUMANOncogene 24p3p25SiderocalinSV40 induced 24P3 protein
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab113326 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Lipocalin-2 / NGAL Human ELISA Kit images

      • ELISA - Lipocalin-2 / NGAL Human ELISA Kit - 1 x 96 wells (ab113326)
        ELISA - Lipocalin-2 / NGAL Human ELISA Kit - 1 x 96 wells (ab113326)

      Protocols

      References for Lipocalin-2 / NGAL Human ELISA Kit (ab113326)

      ab113326 has not yet been referenced specifically in any publications.

      Publishing research using ab113326 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab113326 Submit a Question for ab113326

      Displaying 1 - 6 of 6 results for Abreviews and Q&A

      10
      Votes: Highest First


      What is the shelf life of your assay kits?


      The shelf life of each of our kits is indicated in the individual protocol booklet.

      ab99995 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°...

      Read More

      Hi there,

      Thank you for your help with this enquiry.

      Our customer has responded with the details below:

      1. ab119589 Cystatin C Human ELISA Kit

      a) Could you confirm if the steps under point 7. Preparation was perfo...

      Read More

      Thank you for contacting us.
      It is very important for this assay to dilute samples so that the measurements are in the range of the standard curve.
      The datasheet indicates a range from 4.1 pg/ml - 1000 pg/ml (= 0.041- 1 ng/ml) for the NGAL kit. T...

      Read More

      Hi there,

      One of our customers has been having difficulty detecting proteins of interest with the following ELISA kits- ab119589 and ab113326. Please see below:

      Hi, I recently purchased the Elisa kits for cystatin c and NGAL and am hav...

      Read More

      Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these kits.
      The details you have kindly provided will enable us to investigate this case for yo...

      Read More

      Do you have any idea about the dilution in which the serum or plasma should be applied. I don't think it's mentioned in the protocol.

      Of course we need to test different concentrations, but we want to have an idea about it.

      I am sorry for the delay in getting back to you. I now have further information to share with you in regards to the Lipocalin-2 / NGAL Human ELISA Kit (ab113326).

      I can confirm that we would recommend initially using the kit with serum or plasma ...

      Read More

      As I’m not familiar with these items, I tried to find the ab codes through your site.
      For the items I found a relative code I have update the list.
      For those I don’t have a reference code, this is because either no products matches or ...

      Read More

      I have received information regarding

      ab119589 Cystatin C Human ELISA Kit
      ab119590 Cystatin C Mouse ELISA Kit
      ab119596 KIM1 Mouse ELISA Kit
      ab119597 KIM1 Rat ELISA Kit
      ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
      ab119601 Lipoc...

      Read More

      As I’m not familiar with these items, I tried to find the ab codes through your site.
      For the items I found a relative code I have update the list.
      For those I don’t have a reference code, this is because either no products matches or ...

      Read More

      Thank you for contacting us.


      To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the detai...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"