Lipocalin 2 (NGAL) Human ELISA Kit (ab113326)

Overview

  • Product nameLipocalin 2 (NGAL) Human ELISA KitSee all Lipocalin-2 / NGAL kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.47 69% - 88%
    Serum 103.9 94% - 112%
    Plasma 109.3 96% - 130%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Lipocalin 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Lipocalin 2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Lipocalin 2 coated on a 96- well plate. Standards and samples are pipetted into the wells and Lipocalin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Lipocalin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • Alternative names
    • 24p3
    • 25 kDa alpha 2 microglobulin related subunit of MMP9
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
    • Alpha 2 microglobulin related protein
    • HGNC:6526
    • HNL
    • HNL
    • Lcn 2
    • Lcn2
    • Lipocalin 2
    • Lipocalin-2
    • Migration stimulating factor inhibitor
    • MSFI
    • Neutrophil gelatinase associated lipocalin
    • Neutrophil gelatinase associated lipocalin precursor
    • Neutrophil gelatinase-associated lipocalin
    • NGAL
    • NGAL_HUMAN
    • Oncogene 24p3
    • p25
    • Siderocalin
    • SV40 induced 24P3 protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Lipocalin 2 (NGAL) Human ELISA Kit images

  • Representative Standard Curve using ab113326

Protocols

References for Lipocalin 2 (NGAL) Human ELISA Kit (ab113326)

ab113326 has not yet been referenced specifically in any publications.

Product Wall


The shelf life of each of our kits is indicated in the individual protocol booklet.

ab99995 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80&d...

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It is very important for this assay to dilute samples so that the measurements are in the range of the standard curve.
The datasheet indicates a range from 4.1 pg/ml - 1000 pg/ml (= 0.041- 1 ng/ml) for the NGAL kit...

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I am sorry for the delay in getting back to you. I now have further information to share with you in regards to the Lipocalin-2 / NGAL Human ELISA Kit (ab113326).

I can confirm that we would recommend initially using the kit with serum or plas...

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To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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