You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Lipocalin-2 / NGAL Human ELISA Kit (ab119600)

Submit an Abreview Q&A (2)

Overview

  • Product nameLipocalin-2 / NGAL Human ELISA KitSee all Lipocalin-2 / NGAL kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119600 Lipocalin-2 / NGAL Human ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human NGAL specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NGAL amount of sample captured in plate.

     

    No detectable cross-reactivity with any other cytokine.

  • Notes

    Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

    • Target information above from: UniProt accession P80188 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        24p325 kDa alpha-2-microglobulin-related subunit of MMP-9HNL
        Lcn2Lipocalin 2Lipocalin-2Migration stimulating factor inhibitorMSFINeutrophil gelatinase-associated lipocalinNGALNGAL_HUMANOncogene 24p3p25Siderocalin
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119600 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Lipocalin-2 / NGAL Human ELISA Kit images

      • Sandwich ELISA - Lipocalin-2 / NGAL Human ELISA Kit (ab119600)
        Sandwich ELISA - Lipocalin-2 / NGAL Human ELISA Kit (ab119600)
        Representative standard curve using ab119600.

      Protocols

      References for Lipocalin-2 / NGAL Human ELISA Kit (ab119600)

      ab119600 has not yet been referenced specifically in any publications.

      Publishing research using ab119600 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab119600 Submit a Question for ab119600

      Displaying 1 - 2 of 2 results for Abreviews and Q&A

      10
      Votes: Highest First

      As I’m not familiar with these items, I tried to find the ab codes through your site.
      For the items I found a relative code I have update the list.
      For those I don’t have a reference code, this is because either no products matches or ...

      Read More

      I have received information regarding

      ab119589 Cystatin C Human ELISA Kit
      ab119590 Cystatin C Mouse ELISA Kit
      ab119596 KIM1 Mouse ELISA Kit
      ab119597 KIM1 Rat ELISA Kit
      ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
      ab119601 Lipoc...

      Read More

      As I’m not familiar with these items, I tried to find the ab codes through your site.
      For the items I found a relative code I have update the list.
      For those I don’t have a reference code, this is because either no products matches or ...

      Read More

      Thank you for contacting us.


      To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the detai...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"