Anti-Lipocalin 2 antibody [5G5] (ab23477)

Overview

• Product nameAnti-Lipocalin 2 antibody [5G5]See all Lipocalin 2 primary antibodies ...
• Description
  Mouse monoclonal [5G5] to Lipocalin 2
• Specificityab23477 is specific for Lipocalin 2, native and denatured forms.
• Tested applicationsIHC-P, ELISA, IHC-FoFr, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length native human neutrophil gelatinase associated lipocalin isolated from neutrophils.

• Positive controlCapa-2, BxPC-3, ASPC1 cell line

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number5G5
• Myelomax63-Ag8.653
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Metabolism
  • Types of disease
  • Cancer

  • Cancer
  • Tumor biomarkers
  • Other

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Binding proteins

  • Immunology
  • Adaptive Immunity
  • T Cells
  • Non-CD

Applications

Target

• FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
• Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
• Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
• Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

Target information above from: UniProt accession P80188 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3934 Human
  • Omim: 600181 Human
  • SwissProt: P80188 Human
  • Unigene: 204238 Human

• Alternative names
  24p3 antibody25 kDa alpha 2 microglobulin related subunit of MMP9 antibody25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody

  Alpha 2 microglobulin related protein antibodyHGNC:6526 antibodyHNL antibodyLcn 2 antibodyLcn2 antibodyLipocalin-2 antibodyMigration stimulating factor inhibitor antibodyMSFI antibodyNeutrophil gelatinase associated lipocalin antibodyNeutrophil gelatinase associated lipocalin precursor antibodyNeutrophil gelatinase-associated lipocalin antibodyNGAL antibodyNGAL_HUMAN antibodyOncogene 24p3 antibodyP25 antibodySiderocalin antibodySV40 induced 24P3 protein antibody

  see all

Anti-Lipocalin 2 antibody [5G5] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lipocalin 2 antibody [HYB 211-01] (ab23477)This image is courtesy of an anonymous Abreview

  ab23477 staining human skin tissue sections by IHC-P.  Sections were fixed in 10% neutral buffered formalin and subjected to heat mediated antigen retrieval prior to blocking with 1% BSA for 1 hour at 25°C.  The primary antibody was diluted 1/50 and incubated with the sample for 20 hours at 4°C.  A biotinylated horse anti-mouse antibody diluted 1/200 was used as the secondary.

  See Abreview