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Overview

  • Product nameLipocalin 2 protein (Human)See all Lipocalin 2 proteins and peptides ...
  • Protein descriptionRecombinant full length Human Lipocalin 2; 198 amino acids, 22.6kDa, N-terminal His-tagged. Uniprot ID = P80188.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesFiltered (0.22 µm).
  • FormLyophilised:Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. Aliquot reconstituted protein to avoid repeated freezing /thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • Sequence notesMPLGLLWLGL ALLGALHAQA QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab95007 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Ab95007 can be used as a WB positive control in conjunction with ab41105.
    IP IP: Use at an assay dependent dilution.
    ELISA ELISA: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab31289,ab41105,ab115324 and ab166677.

    • Protein info

    • Alternative names
        24p325 kDa alpha 2 microglobulin related subunit of MMP925 kDa alpha-2-microglobulin-related subunit of MMP-9
        Alpha 2 microglobulin related proteinHGNC:6526HNLLcn 2Lcn2Lipocalin-2Migration stimulating factor inhibitorMSFINeutrophil gelatinase associated lipocalinNeutrophil gelatinase associated lipocalin precursorNeutrophil gelatinase-associated lipocalinNGALNGAL_HUMANOncogene 24p3P25SiderocalinSV40 induced 24P3 protein
      see all
  • FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

    • Target information above from: UniProt accession P80188 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Lipocalin 2 protein (Human) images

    • Western blot
      Western blot
      Anti-Lipocalin 2 antibody (ab41105) at 1 µg/ml + Lipocalin 2 protein (Human) (ab95007) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

    References for Lipocalin 2 protein (Human) (ab95007)

    ab95007 has not yet been referenced specifically in any publications.

    Publishing research using ab95007 ? Please let us know so that we can cite the reference in this datasheet

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