Overview

  • Product nameLiquid Fast-Red Substrate Kit
  • Product overview

    Liquid Fast-Red can be used as a chromogen for immunohistochemical staining and immunoblotting. In the presence of alkaline phosphatase enzyme, Liquid Fast-Red produces a red reaction product that can be seen using either brightfield or fluorescent microscopy.

  • Notes

    Liquid Fast-Red can be used as a chromogen for immunohistochemical staining and immunoblotting. In the presence of alkaline phosphatase enzyme, Liquid Fast-Red produces a red reaction product that can be seen using either brightfield or fluorescent microscopy.

  • Tested applicationsIHC-P, WBmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Storage bufferpH: 8.50
    Constituents: 20% Fast Red TR salt, 20% Naphthol AS-MX phosphate, Tris buffer
  • Components 125 ml
    75x Fast-Red Chromogen 2ml 1 x 2ml
    Napthol Phosphate Substrate Buffer 125ml 1 x 125ml
  • Research Areas
  • RelevanceFast-Red is a widely used chromogen which produces a red reaction product in the presence of alkaline phosphatase enzyme.

Applications

Our Abpromise guarantee covers the use of ab64254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

References for Liquid Fast-Red Substrate Kit (ab64254)

This product has been referenced in:
  • Ceccom J  et al. Reduced sphingosine kinase-1 and enhanced sphingosine 1-phosphate lyase expression demonstrate deregulated sphingosine 1-phosphate signaling in Alzheimer's disease. Acta Neuropathol Commun 2:12 (2014). IHC-P . Read more (PubMed: 24468113) »
  • Srivatsa S  et al. Unc5C and DCC act downstream of Ctip2 and Satb2 and contribute to corpus callosum formation. Nat Commun 5:3708 (2014). IHC-Fr . Read more (PubMed: 24739528) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"