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Liquid Fast-Red Substrate Kit (ab64254)

Q&A (2)

Overview

  • Product nameLiquid Fast-Red Substrate Kit
  • Product overview

    Liquid Fast-Red can be used as a chromogen for immunohistochemical staining and immunoblotting. In the presence of alkaline phosphatase enzyme, Liquid Fast-Red produces a red reaction product that can be seen using either brightfield or fluorescent microscopy.

  • Notes

    Liquid Fast-Red can be used as a chromogen for immunohistochemical staining and immunoblotting. In the presence of alkaline phosphatase enzyme, Liquid Fast-Red produces a red reaction product that can be seen using either brightfield or fluorescent microscopy.

  • Tested applicationsIHC-P, WB more details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Storage bufferpH: 8.50
    Constituents: 20% Fast Red TR salt, 20% Naphthol AS-MX phosphate, Tris buffer
  • Components 125 ml
    75x Fast-Red Chromogen 2ml 1 x 2ml
    Napthol Phosphate Substrate Buffer 125ml 1 x 125ml
  • Research Areas
  • RelevanceFast-Red is a widely used chromogen which produces a red reaction product in the presence of alkaline phosphatase enzyme.

    • Applications

    Our Abpromise guarantee covers the use of ab64254 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P
    WB WB

    References for Liquid Fast-Red Substrate Kit (ab64254)

    ab64254 has not yet been referenced specifically in any publications.

    Publishing research using ab64254 ? Please let us know so that we can cite the reference in this datasheet

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    Substrateis not giving any staining, but previous kits have worked well.

    Thank you for your call today and for letting us know about the trouble with ab64254.

    I have issued credit note# ***, and the full purchase price of ab64524will be refunded to your credit card pending accounts approval. If youhave any questions ...

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    There is a yellow staining after 10 minutes of incubation. No red color.

    Thanks for your call today and for letting us know about the trouble with this kit.

    I'm wondering if you've had a chance to try a longer incubation with the reagent? What were the results? Please feel free to give us a call back if you prefer, o...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"