Listeriolysin (LLO) antibody (ab43018)

Product Name 

Listeriolysin (LLO) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Listeriolysin (LLO)

Immunogen 

Recombinant full length Listeriolysin (LLO) protein

Reacts with 
(species key)

E coli

Specificity 

This antibody will detect the wild type Listeriolysin protein.

Tested applications 
(see key)

ELISA, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1500 - 1/3000.
IP: Use 5µl not diluted.
WB: 1/1000 - 1/1500. Detects a band of approximately 56 kDa (predicted molecular weight: 59 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Listeria

Relevance 

Listeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion.

Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol.
LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.

Listeriolysin O (LLO), a major virulence factor produced by all pathogenic strains of L. monocytogenes.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 0.05% Glycerol

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

The antibody detects the wild type Listeriolysin protein starting from 7 ng in ELISA assay and 15 ng in Western- immunoblotting assay.

See below for Listeriolysin (LLO) antibody images, references, products related to ab43018 and other tools.

Listeriolysin (LLO) antibody images:

References for Listeriolysin (LLO) antibody (ab43018)

  This product has been used in: (two most recent references)

Giese B et al. Staphylococcal alpha-toxin is not sufficient to mediate escape from phagolysosomes in upper-airway epithelial cells. Infect Immun 77:3611-25 (2009). PubMed: 19564384

Lehrer RI et al. Human alpha-defensins inhibit hemolysis mediated by cholesterol-dependent cytolysins. Infect Immun 77:4028-40 (2009). PubMed: 19581399

See all 3 publication references for this product.


If you publish research using ab43018 please let us know so that we can cite the reference on this datasheet.

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