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Products:Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Listeria
Overview
Product name
Anti-Listeriolysin (LLO) antibody
Description
Rabbit polyclonal to Listeriolysin (LLO)
Specificity
This antibody will detect the wild type Listeriolysin protein.
Tested applications
WB, ELISA, IPmore details
Cross reactivity
Reacts with
Escherichia coli , Listeria monocytogenes
Immunogen
Recombinant full length Listeriolysin (LLO) protein
General notes
The antibody detects the wild type Listeriolysin protein starting from 7 ng in ELISA assay and 15 ng in Western- immunoblotting assay.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.05% Glycerol
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
Listeriolysin O (LLO), a major virulence factor produced by all pathogenic strains of L. monocytogenes.
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Listeria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab43018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/1500.Detects a band of approximately 56 kDa (predicted molecular weight: 59 kDa).
ELISA: 1/1500 - 1/3000.
IP: Use at an assay dependent dilution. (Use 5µl not diluted.)
Target
Relevance
Listeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.
Alternative names
- hly antibody
- hlyA antibody
- lisA antibody
see all
Anti-Listeriolysin (LLO) antibody images:
Western blot - Listeriolysin (LLO) antibody (ab43018)
Anti-Listeriolysin (LLO) antibody (ab43018) at 1/1000 dilution
Predicted band size : 59 kDa
ELISA - Listeriolysin (LLO) antibody (ab43018)
ab43018 detecting Listeriolysin in Listeria monocytogenes samples by indirect ELISA. Samples were blocked with 5% milk for 1 hour at 20°C and incubated with the primary antibody (1/3000 in TBS + 0.1% Tween-20) for 1 hour at 20°C. An alkaline phosphatase Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-Listeriolysin (LLO) antibody (ab43018)
This product has been referenced in:
- Vadia Set al. The pore-forming toxin listeriolysin O mediates a novel entry pathway of L. monocytogenes into human hepatocytes. PLoS Pathog 7:e1002356 (2011). WB; Listeria monocytogenes.Read more (PubMed: 22072970) »
- Giese Bet al. Staphylococcal alpha-toxin is not sufficient to mediate escape from phagolysosomes in upper-airway epithelial cells. Infect Immun 77:3611-25 (2009).Read more (PubMed: 19564384) »
See all 3 publications for this product
Publishing research using ab43018? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"