Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Listeria
Overview
Product name
Listeriolysin (LLO) protein (Active)
See all Listeriolysin (LLO) products (3) ...
Protein description
Recombinant fragment, corresponding to amino acids 60-529 ( 471 amino acids ), of Listeriolysin (LLO) lacking both the signal secretion sequence and the PEST-like sequence, with the addition of a methionine in its NH2-terminus. Predicted M.Wt 55 kDa
Expression host
E. coli
Properties
Purity
>90% by SDS-PAGE
Purification notes
Purity: Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE
Biological activity
Activity : 1.25x106 HU/mg. 2 mM DTT could be use to reactivate the toxin.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 5% Glycerol, 0.5M Sodium chloride, 50mM Sodium dihydrogen phosphate, 2.7mM Potassium chloride, 1mM DTT, 1mM EDTA
Concentration
Concentration information loading...
Research areas
Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Listeria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Relevance
Listeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.
References for Listeriolysin (LLO) protein (Active) (ab68200)
ab68200 has not yet been referenced specifically in any publications.
Publishing research using ab68200? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"