Overview
Product nameListeriolysin (LLO) protein (Active)See all Listeriolysin (LLO) proteins and peptides ...
Protein descriptionRecombinant fragment, corresponding to amino acids 60-529 ( 471 amino acids ), of Listeriolysin (LLO) lacking both the signal secretion sequence and the PEST-like sequence, with the addition of a methionine in its NH2-terminus. Predicted M.Wt 55 kDa
Expression hostE. coli
Properties
Purity> 90
% by SDS-PAGE
Purification notesPurity: Greater than 90.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE
Biological activityActivity : 1.25x106 HU/mg. 2 mM DTT could be use to reactivate the toxin.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 5% Glycerol, 0.5M Sodium chloride, 50mM Sodium dihydrogen phosphate, 2.7mM Potassium chloride, 1mM DTT, 1mM EDTA
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab68200
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
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Alternative names
LLOThiol activated cytolysin
see all
RelevanceListeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion.
Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol.
LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.
References for Listeriolysin (LLO) protein (Active) (ab68200)
ab68200
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
