Listeriolysin (LLO) protein (Active) (ab68200)

Product Name 

Listeriolysin (LLO) protein (Active)

Product type 

Proteins and Peptides

Peptide 

Recombinant fragment, corresponding to amino acids 60-529 ( 471 amino acids ), of Listeriolysin (LLO) lacking both the signal secretion sequence and the PEST-like sequence, with the addition of a methionine in its NH2-terminus. Predicted M.Wt 55 kDa

Specificity 

Activity : 1.25x10⁶ HU/mg. 2 mM DTT could be use to reactivate the toxin.

Tested applications 
(see key)

SDS-PAGE

Application notes 
(see key)

Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Listeria

Relevance 

Listeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion.

Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol.
LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.

This protein can be used for: Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA.

Purity 

>90% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 5% Glycerol, 0.5M Sodium chloride, 50mM Sodium dihydrogen phosphate, 2.7mM Potassium chloride, 1mM DTT, 1mM EDTA

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Phosphate MSDS

Purification notes 

Purity: Greater than 90.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE

Form 

Liquid

Concentration 

Batch dependent within range: 0.70 - 0.70 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Expression system 

E. coli

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