Listeriolysin (LLO) protein (Active) (ab83345)

Product Name 

Listeriolysin (LLO) protein (Active)

Product type 

Proteins and Peptides

Peptide 

Recombinant fragment, corresponding to amino acids 60-529 of Listeriolysin (LLO) lacking both the signal secretion sequence and the PEST-like sequence (Listeria monocytogenes), with the addition of a methionine in its NH2-terminus. MW 52.5 kDa, Swiss Prot P13128.

Specificity 

Hemolytic activity: 1.25x10⁶ HU/mg. 2 mM DTT could be use to reactivate the toxin.

Tested applications 
(see key)

FuncS, SDS-PAGE

Application notes 
(see key)

Recommended dilutions
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Listeria

Relevance 

Listeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion.

Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol.
LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.

Purity 

Ion Exchange Chromatography

Storage buffer 

Preservative: None
Constituents: 5% Glycerol, 0.5M Sodium chloride, 50mM Sodium phosphate, 2.7mM Potassium chloride, 1mM DTT, 1mM EDTA

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Phosphate MSDS

Purification notes 

Purified by hydroxyapatite and ion-exchange chromatography

Form 

Liquid

Concentration 

0.700 mg/ml

Storage instructions 

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Expression system 

E. coli

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