Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Live/Dead Cell Assay
See all Live dead cell products (2) ...
Product overview
ab115347 is a one-step assay to differentially labels live and dead cells with fluorescent dyes. It is useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy. The provided Live/Dead Assay stain is sufficient for ~1000 assays.
The Live/Dead assay stain solution is a mixture of two highly fluorescent dyes that differentially label live and dead cells:
The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm, respectively (similar to FITC).
The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm, respectively.
The Live/Dead Cell Assay is a one-step staining procedure that is simple and fast. It can be used directly in cell culture media. Stained cells are not compatible with fixation.
Notes
Store the Live/Dead staining solution at 4°C in the dark. The
product is stable for 6 months from receipt. For longer term storage,
aliquot and store at -20°C or -80°C. Avoid multiple freeze-thaw
cycles. Allow the product to warm to room temperature before
opening. Use diluted solutions of Live/Dead stain promptly.
product is stable for 6 months from receipt. For longer term storage,
aliquot and store at -20°C or -80°C. Avoid multiple freeze-thaw
cycles. Allow the product to warm to room temperature before
opening. Use diluted solutions of Live/Dead stain promptly.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Please see Notes section
| Components | 1000 tests |
|---|---|
| 1000X Live/Dead Cell stain in DMSO | 1 x 0.1ml |
Research areas
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits >> Cell viability, plasma membrane damage
Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Other Apoptosis Kits
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits
Relevance
Distinguishing between live and dead cells is very important for investigation of growth control and cell death.
Alternative names
- MS961
Applications
Show applications key
Our Abpromise guarantee covers the use of ab115347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
Live/Dead Cell Assay images:
Functional Studies - Live/Dead Cell Assay (ab115347)
Jurkat cells were treated with a dose response of Etoposide
on day 0 and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry. At each time point a small aliquot of cells was removed from the culture for analysis.
(A) Dot plots showing live/dead analysis of day 3 samples. Live cells are on the y-axis and dead cells are on the x-axis. The red polygongate identifies live cells and the number indicates the percent of live cells.
Functional Studies - Live/Dead Cell Assay (ab115347)
Flow cytometry analysis using the Live/Dead assay stain. Jurkat cells were treated with a dose response of Etoposide on day 0 and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry. At each time point a small aliquot of cells was removed from the culture for analysis.
Graphical representation of the data presented in (A) with additional time points. % viable cells are plotted relative to time, with respect to Etoposide concentration.
Functional Studies - Live/Dead Cell Assay (ab115347)
The sample dot plots demonstrate varying ratios of live and dead cells.
Functional Studies - Live/Dead Cell Assay (ab115347)
Jurkat cells stained with the live/dead assay kit. Jurkat cells treated with Etoposide were labeled with the live/dead assay stain. Live cells (with esterase activity) stain green and dead cells (compromised plasma membrane) stain red. (A) Field of cells following 10 minute staining in media of live/dead stain. (B)Magnified view showing that in live cells the whole cell is stained green whereas in dead red cells it is the fragmented nuclear DNA that is stained.
Protocols
References for Live/Dead Cell Assay (ab115347)
ab115347 has not yet been referenced specifically in any publications.
Publishing research using ab115347? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"